未麻醉微型猪脑脊液中褪黑素浓度的日变化
Diurnal Variation of Melatonin Concentration in the Cerebrospinal Fluid of Unanesthetized Microminipig.
作者信息
Abe Masaharu, Kawaguchi Hiroaki, Miura Naoki, Akioka Kohei, Ushikai Miharu, Oi Sayumi, Yukawa Airo, Yoshikawa Tetsuya, Izumi Hiroyuki, Horiuchi Masahisa
机构信息
Department of Hygiene and Health Promotion Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan.
Department of Hygiene and Health Promotion Medicine, Kagoshima University Graduate School of Medical and Dental Sciences, Kagoshima, Japan
出版信息
In Vivo. 2018 May-Jun;32(3):583-590. doi: 10.21873/invivo.11279.
BACKGROUND/AIM: The aim of this study was to develop a method for sequentially collecting cerebrospinal fluid (CSF) from an unanesthetized microminipig, which shares many physiological and anatomical similarities with humans, such as diurnality, and investigate the diurnal variation of melatonin concentration in the CSF.
MATERIALS AND METHODS
A catheter was placed percutaneously into the subarachnoid space of an anesthetized animal, and the tip of the catheter was placed into the cisterna magna under X-ray. We then sequentially collected CSF at light-on and -off times from the unanesthetized animal for several weeks. After catheter placement, a period of one week or more was necessary to relieve the contamination of RBCs in the CSF.
RESULTS
A higher melatonin level in the CSF was noted during lights-off time, and the level was higher than that in the serum.
CONCLUSION
This model of sequential collection of CSF will contribute to research in brain functions.
背景/目的：本研究的目的是开发一种从未麻醉的小型微型猪中顺序采集脑脊液（CSF）的方法，这种猪在许多生理和解剖学方面与人类相似，如昼夜节律，并研究脑脊液中褪黑素浓度的昼夜变化。
材料与方法
将一根导管经皮插入麻醉动物的蛛网膜下腔，并在X射线引导下将导管尖端置于枕大池。然后，我们在数周内从未麻醉的动物身上，在光照开启和关闭时顺序采集脑脊液。放置导管后，需要一周或更长时间来减少脑脊液中红细胞的污染。
结果
在熄灯期间，脑脊液中褪黑素水平较高，且该水平高于血清中的水平。
结论
这种顺序采集脑脊液的模型将有助于脑功能的研究。
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