Morozova V, Kozlova Yu, Shedko E, Babkin I, Kurilshikov A, Bokovaya O, Bardashova A, Yunusova A, Tikunov A, Tupikin A, Ushakova T, Ryabchikova E, Tikunova N
Institute of Chemical Biology and Fundamental Medicine SB RAS, Lavrentieva Ave. 8, Novosibirsk, 630090, Russia.
Novosibirsk State University, Pirogova str. 1, Novosibirsk, 630090, Russia.
Arch Virol. 2018 Aug;163(8):2189-2197. doi: 10.1007/s00705-018-3853-3. Epub 2018 May 2.
Four lytic Proteus bacteriophages, PM75, PM85, PM93, and PM116, which are active against multi-drug-resistant strains of P. mirabilis, were isolated from cattle and poultry samples. According to electron microscopy data, all of the investigated phages belonged to the family Podoviridae. They all demonstrated lytic activity against sensitive strains of P. mirabilis, and three of the phages, PM85, PM93, and PM116, are potential candidates for use in antibacterial treatment. The genomes and putative proteins of bacteriophages PM85, PM93, and PM116 were similar to those of Proteus phage vB_PmiP_Pm5460 [KP890822], and the investigated phages formed a distinct clade within the genus Sp6virus, subfamily Autographivirinae. The genome sequence of phage PM75 was similar to that of a previously described Proteus phage, PM16 [KF319020], and both of them demonstrated low nucleotide sequence identity to the genomes of the other most similar phages, namely, Vibrio phage VP93, Pantoea phage LIMElight, and KP34-like bacteriophages. According to cluster analysis of the complete genome sequences and phylogenetic analysis of the proteins essential for their life cycle, phages PM75 and PM16 are distinct from other similar phages from the phiKMV supergroup and should be recognized as constituting a new genus, "Pm16virus", within the subfamily Autographivirinae.
从牛和家禽样本中分离出了四种裂解性变形杆菌噬菌体，即PM75、PM85、PM93和PM116，它们对奇异变形杆菌的多重耐药菌株具有活性。根据电子显微镜数据，所有被研究的噬菌体都属于短尾噬菌体科。它们都对奇异变形杆菌的敏感菌株表现出裂解活性，其中三种噬菌体，PM85、PM93和PM116，是抗菌治疗的潜在候选者。噬菌体PM85、PM93和PM116的基因组和推定蛋白与变形杆菌噬菌体vB_PmiP_Pm5460 [KP890822]相似，并且所研究的噬菌体在Sp6病毒属、自剪接病毒亚科内形成了一个独特的分支。噬菌体PM75的基因组序列与先前描述的变形杆菌噬菌体PM16 [KF319020]相似，并且它们两者与其他最相似噬菌体的基因组，即弧菌噬菌体VP93、泛菌噬菌体LIMElight和KP3类噬菌体，显示出低核苷酸序列同一性。根据完整基因组序列的聚类分析和其生命周期必需蛋白的系统发育分析，噬菌体PM75和PM16与phiKMV超群的其他相似噬菌体不同，应被认定为在自剪接病毒亚科内构成一个新属，即“Pm16病毒属”。
