Hua Li, Shi Jiayuan, Shultz Leonard D, Ren Guangwen
The Jackson Laboratory, Bar Harbor, ME, USA.
Methods Mol Biol. 2018;1784:243-258. doi: 10.1007/978-1-4939-7837-3_22.
Macrophages are a heterogeneous population of innate immune cells and are distributed in most adult tissues. Certain tissue-resident macrophages with a prenatal origin, together with postnatal monocyte-derived macrophages, serve as the host scavenger system to eliminate invading pathogens, malignant cells, senescent cells, dead cells, cellular debris, and other foreign substances. As a key member of the mononuclear phagocyte system, macrophages play essential roles in regulation of prenatal development, tissue homeostasis, and disease progression. Over the past two decades, considerable efforts have been made to generate genetic models of macrophage ablation in mice. These models support investigations of the precise functions of tissue-specific macrophages under physiological and pathological conditions. Herein, we overview the currently available mouse strains for in vivo genetic ablation of macrophages and discuss their respective advantages and limitations.
巨噬细胞是先天性免疫细胞的异质性群体，分布于大多数成年组织中。某些起源于产前的组织驻留巨噬细胞，与产后单核细胞衍生的巨噬细胞一起，作为宿主清除系统，以清除入侵的病原体、恶性细胞、衰老细胞、死亡细胞、细胞碎片和其他外来物质。作为单核吞噬细胞系统的关键成员，巨噬细胞在产前发育、组织稳态和疾病进展的调节中发挥着重要作用。在过去二十年中，人们为建立小鼠巨噬细胞消融的遗传模型付出了巨大努力。这些模型有助于研究生理和病理条件下组织特异性巨噬细胞的精确功能。在此，我们概述了目前可用于体内巨噬细胞基因消融的小鼠品系，并讨论了它们各自的优缺点。
