Doctorate in Biosciences, School of Engineering, Universidad de La Sabana, Chía 250001, Colombia.
Bioprospecting Research Group, School of Engineering, Universidad de La Sabana, Chía 250001, Colombia.
Mar Drugs. 2021 Nov 27;19(12):674. doi: 10.3390/md19120674.
Sunscreen and sunblock are crucial skincare products to prevent photoaging and photocarcinogenesis through the addition of chemical filters to absorb or block ultraviolet (UV) radiation. However, several sunscreen and sunblock ingredients, mostly UV filters, have been associated with human and environmental safety concerns. Therefore, the exploration and discovery of promising novel sources of efficient and safer compounds with photoprotection-related activities are currently required. Marine invertebrates, particularly their associated microbiota, are promising providers of specialized metabolites with valuable biotechnological applications. Nevertheless, despite Actinobacteria members being a well-known source of bioactive metabolites, their photoprotective potential has been poorly explored so far. Hence, a set of methanolic extracts obtained from -derived actinomycetes was screened regarding their antioxidant and UV-absorbing capacities (i.e., photoprotection-related activities). The active extract-producing strains were identified and classified within genera and This is the first report of the isolation of these microorganisms from (an ecologically important Caribbean coral reef-boring sponge). The in vitro cytotoxicity on dermal fibroblasts of oxybenzone and the selected active extracts revealed that oxybenzone exerted a cytotoxic effect, whereas no cytotoxic effect of test extracts was observed. Accordingly, the most active (SPFi > 5, radical scavenging > 50%) and nontoxic (cell viability > 75%) extracts were obtained from strains. Finally, LC-MS-based characterization suggested a broad chemical space within the test strains and agreed with the reported streptomycetes' chemodiversity. The respective metabolite profiling exposed a strain-specific metabolite occurrence, leading to the recognition of potential hits. These findings suggest that marine produce photoprotectants ought to be further explored in skincare applications.
防晒霜和防晒乳是预防光老化和光致癌的关键护肤品，通过添加化学过滤器来吸收或阻挡紫外线 (UV) 辐射。然而，一些防晒霜和防晒乳成分，主要是紫外线过滤器，与人类和环境安全问题有关。因此，目前需要探索和发现具有防晒相关活性的有前途的新型高效和更安全化合物的来源。海洋无脊椎动物，特别是它们相关的微生物群，是具有有价值生物技术应用的特殊代谢物的有前途的提供者。然而，尽管放线菌成员是生物活性代谢物的已知来源，但迄今为止，它们的防晒潜力尚未得到充分探索。因此，一组从 衍生的放线菌获得的甲醇提取物就其抗氧化和紫外线吸收能力（即，与防晒相关的活性）进行了筛选。对产生活性提取物的菌株进行了鉴定和分类，属于 和 属。这是首次从 （加勒比生态重要的珊瑚礁钻孔海绵）中分离出这些微生物的报道。对氧苯酮和选定的活性提取物的真皮成纤维细胞的体外细胞毒性表明，氧苯酮具有细胞毒性作用，而测试提取物没有观察到细胞毒性作用。因此，从 菌株中获得了最活跃的（SPFi > 5，自由基清除 > 50%）和无毒的（细胞活力 > 75%）提取物。最后，基于 LC-MS 的表征表明测试菌株具有广泛的化学空间，与报道的链霉菌的化学多样性一致。各自的代谢产物分析揭示了测试菌株中存在特定菌株的代谢产物，从而识别出潜在的命中物。这些发现表明，海洋 产生的防晒剂应该在护肤应用中进一步探索。
