Shi Donghui, Zhou Jianchuan, Zhao Lihong, Rong Xiaoping, Fan Yu, Hamid Humera, Li Wenqiang, Ji Cheng, Ma Qiugang
1State Key Laboratory of Animal Nutrition, China Agricultural University, Beijing, 100193 People's Republic of China.
2Liaoning Medical University, Jinzhou, 121001 People's Republic of China.
J Anim Sci Biotechnol. 2018 May 16;9:42. doi: 10.1186/s40104-018-0255-z. eCollection 2018.
The current study was carried out to evaluate the effects of mycotoxin biodegradation agent (MBA, composed of ANSB01G and sp. ANSB714) on relieving zearalenone (ZEA) and deoxynivalenol (DON) toxicosis in immature gilts.
A total of forty pre-pubertal female gilts (61.42 ± 1.18 kg) were randomly allocated to four diet treatments: CO (positive control); MO (negative control, ZEA 596.86 μg/kg feed and DON 796 μg/kg feed); COA (CO + 2 g MBA/kg feed); MOA (MO + 2 g MBA/kg feed). Each treatment contained 10 replicates with 1 gilt per replicate. Gilts were housed in an environmentally controlled room with the partially slatted floor.
During the entire experimental period of 28 d, average daily gain (ADG) and average daily feed intake (ADFI) of gilts in MO group was significantly reduced compared with those in CO group. The vulva size of gilts was significantly higher in MO group than CO group. In addition, significant increases in the plasma levels of IgA, IgG, IL-8, IL-10 and PRL were determined in MO group compared with that in CO group. ZEA and DON in the diet up-regulated apoptotic caspase-3 in ovaries and uteri, along with down-regulated the anti-apoptotic protein Bcl-2 in ovaries. The supplementation of MBA into diets co-contaminated with ZEA and DON significantly increased ADG, decreased the vulva sizes, reduced the levels of IgG, IL-8 and PRL in plasma, and regulated apoptosis in ovaries and uteri of gilts.
The present results indicated that feeding diet contaminated with ZEA and DON simultaneously (596.86 μg/kg + 796 μg/kg) had detrimental effects on growth performance, plasma immune function and reproductive status of gilts. And MBA could reduce the negative impacts of these two toxins, believed as a promising feed additive for mitigating toxicosis of ZEA and DON at low levels in gilts.
本研究旨在评估霉菌毒素生物降解剂（MBA，由ANSB01G和ANSB714菌株组成）对缓解未成熟后备母猪玉米赤霉烯酮（ZEA）和脱氧雪腐镰刀菌烯醇（DON）中毒的效果。
总共40头青春期前的后备母猪（61.42±1.18千克）被随机分配到四种日粮处理组：CO（阳性对照）；MO（阴性对照，日粮中ZEA含量为596.86微克/千克，DON含量为796微克/千克）；COA（CO + 2克MBA/千克日粮）；MOA（MO + 2克MBA/千克日粮）。每个处理组包含10个重复，每个重复1头母猪。母猪饲养在环境可控的房间内，地面部分为漏缝地板。
在整个28天的实验期内，MO组母猪的平均日增重（ADG）和平均日采食量（ADFI）与CO组相比显著降低。MO组母猪的外阴尺寸显著大于CO组。此外，与CO组相比，MO组血浆中IgA、IgG、IL-8、IL-10和PRL水平显著升高。日粮中的ZEA和DON上调了卵巢和子宫中凋亡相关蛋白caspase-3的表达，同时下调了卵巢中抗凋亡蛋白Bcl-2的表达。在被ZEA和DON共同污染的日粮中添加MBA可显著提高ADG，减小外阴尺寸，降低血浆中IgG、IL-8和PRL水平，并调节后备母猪卵巢和子宫中的细胞凋亡。
目前的结果表明，同时饲喂被ZEA和DON污染的日粮（596.86微克/千克 + 796微克/千克）对后备母猪的生长性能、血浆免疫功能和生殖状态有不利影响。而MBA可以减少这两种毒素的负面影响，被认为是一种有前景的饲料添加剂，可减轻后备母猪低水平ZEA和DON中毒。
