Department of Biochemistry and Molecular Biology, University of Bucharest, Bucharest, Romania.
Departament of Toxicology, Faculty of Pharmacy, "Carol Davila" University of Medicine and Pharmacy, Bucharest, Romania.
Cartilage. 2020 Jan;11(1):88-97. doi: 10.1177/1947603518775798. Epub 2018 May 29.
Cetylated fatty acids are a group of naturally occurring fats of plant and/or animal origin. Cetyl myristoleate, in particular, was initially involved in osteoarthritis related research as its therapeutic administration prevented experimentally induced arthritis in Swiss Albino mice. In this context, the aim of our study was to investigate the possible mechanisms of Celadrin cetylated fatty acids action at the cellular level inflammation related pain relief and chondrogenesis.
For this, we tested the effects of the cetylated fatty acids mixture from Celadrin on an scaffold-free 3-dimensional mesenchymal stem cells culture model of chondrogenesis. Furthermore, we treated stimulated mouse macrophage cells with the cetylated fatty acids mixture to investigate the expression profile of secreted inflammatory cytokines.
The cetylated fatty acids mixture from Celadrin significantly decreased the production of IL-6, MCP-1, and TNF, key regulators of the inflammatory process, in stimulated RAW264.7 mouse macrophage cells. The treatment with cetylated fatty acids mixture initiated and propagated the process of chondrogenesis as demonstrated by the increased expression and deposition of chondrogenic markers by the differentiating mesenchymal cells.
The cetylated fatty acids mixture from Celadrin reduces inflammation by significantly decreasing the expression of IL-6, MCP-1, and TNF in stimulated RAW264.7 mouse macrophage cells. These compounds facilitate the chondrogenic differentiation process of human adipose-derived stem cells by stimulating the expression of chondrogenic markers under chondrogenic induction conditions.
乙酰化脂肪酸是一组天然存在的植物和/或动物脂肪。特别是肉豆蔻酸己酯，最初作为治疗药物参与骨关节炎相关研究，因为它的治疗给药可预防瑞士白化病小鼠实验性诱导关节炎。在这种情况下，我们研究的目的是研究 Celadrin 乙酰化脂肪酸在细胞水平缓解炎症相关疼痛和软骨发生方面的可能作用机制。
为此，我们测试了 Celadrin 中的乙酰化脂肪酸混合物对无支架 3 维间充质干细胞软骨发生培养模型的影响。此外，我们用乙酰化脂肪酸混合物处理刺激的小鼠巨噬细胞，以研究分泌的炎症细胞因子的表达谱。
Celadrin 中的乙酰化脂肪酸混合物显著降低了刺激的 RAW264.7 小鼠巨噬细胞中白细胞介素 6（IL-6）、单核细胞趋化蛋白 1（MCP-1）和肿瘤坏死因子（TNF）的产生，这些细胞因子是炎症过程的关键调节因子。用乙酰化脂肪酸混合物处理可启动和促进软骨发生过程，这表现为分化间充质细胞表达和沉积更多的软骨生成标志物。
Celadrin 中的乙酰化脂肪酸混合物通过显著降低刺激的 RAW264.7 小鼠巨噬细胞中 IL-6、MCP-1 和 TNF 的表达来减轻炎症。这些化合物通过在软骨形成诱导条件下刺激软骨生成标志物的表达，促进人脂肪来源干细胞的软骨生成分化过程。
