大肠杆菌K-12中crp基因表达的调控：体内研究
Regulation of expression of the crp gene of Escherichia coli K-12: in vivo study.
作者信息
Cossart P, Gicquel-Sanzey B
出版信息
J Bacteriol. 1985 Jan;161(1):454-7. doi: 10.1128/jb.161.1.454-457.1985.
Abstract
Expression of the crp gene was studied in vivo by use of a crp-lacZ gene fusion first constructed on a plasmid and then transferred onto the chromosome. Our in vivo data confirm the in vitro findings that crp is negatively autoregulated via the cyclic AMP-catabolite gene activator protein complex. We present evidence that gene crp is repressed by glucose.
摘要
通过使用首先构建在质粒上然后转移到染色体上的crp-lacZ基因融合体，在体内研究了crp基因的表达。我们的体内数据证实了体外研究结果，即crp通过环磷酸腺苷-分解代谢物基因激活蛋白复合物进行负向自我调节。我们提供证据表明crp基因受到葡萄糖的抑制。
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