Ito M, Ihara T, Grose C, Starr S
J Virol. 1985 Apr;54(1):98-103. doi: 10.1128/JVI.54.1.98-103.1985.
Seven murine monoclonal antibodies reacting with major glycoproteins of varicella-zoster virus were tested for functional activity in assays for antibody-dependent cellular cytotoxicity (ADCC) and antibody-plus-complement-mediated lysis. Human peripheral blood mononuclear cells killed varicella-zoster virus-infected fibroblasts in the presence of three of four monoclonal antibodies directed against gp98/62 and a single monoclonal antibody directed against gp118. Neither of two monoclonal antibodies directed against gp66 was able to mediate ADCC. In 18-h assays, adherent effector cells were more active than nonadherent effector cells in mediating ADCC. Adherent cells treated with anti-Leu-11b and complement retained their cytotoxic activity, suggesting that monocytes are responsible for most of the adherent-cell-mediated cytotoxicity. Both immunoglobulin G1 and G2a murine monoclonal antibodies were able to participate in ADCC. Of the two immunoglobulin G2a monoclonal antibodies tested, both of which reacted with gp98/62, only one mediated lysis in the presence of complement. These results indicate that some murine monoclonal antibodies against major glycoproteins of varicella-zoster virus have functional activity in cytotoxicity assays.
对七种与水痘-带状疱疹病毒主要糖蛋白发生反应的鼠单克隆抗体进行了检测，以评估其在抗体依赖性细胞毒性（ADCC）和抗体加补体介导的裂解试验中的功能活性。在针对gp98/62的四种单克隆抗体中的三种以及针对gp118的一种单克隆抗体存在的情况下，人外周血单个核细胞杀死了水痘-带状疱疹病毒感染的成纤维细胞。针对gp66的两种单克隆抗体均不能介导ADCC。在18小时的试验中，贴壁效应细胞在介导ADCC方面比非贴壁效应细胞更具活性。用抗Leu-11b和补体处理的贴壁细胞保留了其细胞毒性活性，这表明单核细胞是大多数贴壁细胞介导的细胞毒性的原因。免疫球蛋白G1和G2a鼠单克隆抗体均能够参与ADCC。在测试的两种与gp98/62发生反应的免疫球蛋白G2a单克隆抗体中，只有一种在补体存在的情况下介导裂解。这些结果表明，一些针对水痘-带状疱疹病毒主要糖蛋白的鼠单克隆抗体在细胞毒性试验中具有功能活性。
