Department of Bioanalysis, Faculty of Pharmaceutical Sciences, Ghent University, Ghent, Belgium.
Department of Food Safety and Food Quality, Faculty of Bioscience Engineering, Ghent University, Ghent, Belgium.
J Sci Food Agric. 2019 Jan 15;99(1):253-262. doi: 10.1002/jsfa.9167. Epub 2018 Jul 20.
Fusarium head blight (FHB) is a well-known disease of wheat caused by a complex of Fusarium species. In this research, an extensive study on the occurrence of the emerging Fusarium cyclodepsipeptide mycotoxins beauvericin and enniatins was conducted in Belgian wheat grains harvested in 2015 and 2016. To assess the link between Fusarium species and their mycotoxin production, ultra-performance liquid chromatography-tandem mass spectrometry was used to quantify the cyclodepsipeptide mycotoxins, while quantitative polymerase chain reaction was applied to quantify the presence of Fusarium species.
It was shown that enniatins were mainly associated with the presence of F. avenaceum, while beauvericin, despite its low incidence, correlated significantly with F. poae. The application of fungicides resulted in a species shift and in the occurring mycotoxins. Concerning the effect of weather conditions, it was seen that levels of enniatins were positively correlated with the rainfall in May and June, while a negative correlation was observed with rainfall in the first half of July.
Our study provides new insights into the occurrence of the emerging cyclodepsipeptide mycotoxins in an agro-ecosystem in which fungicides are the main control measure against FHB. It seems that beauvericin and enniatin levels are affected by different parameters and behave differently upon application of fungicides. © 2018 Society of Chemical Industry.
镰刀菌穗腐病（FHB）是一种由镰刀菌属真菌引起的小麦知名病害。在这项研究中，对 2015 年和 2016 年收获的比利时小麦籽粒中新兴的环二肽类霉菌毒素 beauvericin 和 enniatins 的发生情况进行了广泛研究。为了评估镰刀菌属与其霉菌毒素产生之间的联系，采用超高效液相色谱-串联质谱法对环二肽类霉菌毒素进行定量，同时应用定量聚合酶链式反应对镰刀菌属的存在进行定量。
结果表明，enniatins 主要与 F. avenaceum 的存在相关，而 beauvericin 尽管发病率较低，但与 F. poae 显著相关。杀菌剂的应用导致了物种转移和发生的霉菌毒素。关于气象条件的影响，观察到 enniatins 的水平与 5 月和 6 月的降雨量呈正相关，而与 7 月上半月的降雨量呈负相关。
我们的研究为在杀菌剂是防治 FHB 的主要控制措施的农业生态系统中环二肽类新兴霉菌毒素的发生提供了新的见解。似乎 beauvericin 和 enniatin 的水平受不同参数的影响，并且在应用杀菌剂时表现不同。 © 2018 英国化学学会。
