Kengne Michel, Shu Siri Vivian, Nwobegahay Julius Mbekem, Achonduh Olivia
Medical Microbiology and Immunology Department, School of Health Sciences, Catholic University of Central Africa, Yaoundé, Cameroon.
Bethesda Hospital, Yaoundé, Cameroon.
Pan Afr Med J. 2017 Dec 6;28:294. doi: 10.11604/pamj.2017.28.294.11200. eCollection 2017.
Vaginal candidiasis is considered as an important public health problem worldwide and its incidence has increased nowadays. In recent years, inappropriate and disproportionate use of antifungal drugs, automedication as well as non compliance have caused drug resistance.
This study aimed at determining the antifungal susceptibility patterns of isolated from female genital tract at in Cameroon. Two hundred and fourthy five women (age range: 15 years to 49 years) attending the hospital were recruited between January and June 2014 in this cross sectional study. Vaginal smears were collected using sterile swabs from each participant and cultured on sabouraud dextrose agar supplemented with chloramphenico l 0.5%; identification of was performed following standard methods. The disk diffusion method was used for antifungal susceptibility testing.
Out of the 245 vaginal smears collected, 94 (38.4%) strains of yeast were isolates among which 43 (45.7%) were and 51 (54.3%) were non albicans. The highest susceptibility of the isolates was seen for nystatin 62 (83.78%), ketoconazole 61 (82.43%) and fluconazole 60 (81.08%).
Despite the noticeable resistance of isolates to miconazole and itraconazole, the results indicate that nystatin, ketoconazole and fluconazole are the drugs of choice for the therapy of vaginal candidiasis in this region.
阴道念珠菌病被视为全球重要的公共卫生问题，如今其发病率有所上升。近年来，抗真菌药物的不当和过度使用、自行用药以及不依从性导致了耐药性。
本研究旨在确定喀麦隆某医院分离出的女性生殖道念珠菌的抗真菌药敏模式。在2014年1月至6月期间，招募了245名到该医院就诊的女性（年龄范围：15岁至49岁）参与这项横断面研究。使用无菌拭子从每位参与者采集阴道涂片，并在添加了0.5%氯霉素的沙氏葡萄糖琼脂上培养；按照标准方法进行念珠菌的鉴定。采用纸片扩散法进行抗真菌药敏试验。
在收集的245份阴道涂片中，分离出94株酵母菌菌株，其中43株（45.7%）为白色念珠菌，51株（54.3%）为非白色念珠菌。分离菌株对制霉菌素的敏感性最高，为62株（83.78%），酮康唑为61株（82.43%），氟康唑为60株（81.08%）。
尽管白色念珠菌分离株对咪康唑和伊曲康唑有明显耐药性，但结果表明制霉菌素、酮康唑和氟康唑是该地区治疗阴道念珠菌病的首选药物。
