Liang Wenxuan, Hall Gunnsteinn, Messerschmidt Bernhard, Li Ming-Jun, Li Xingde
Department of Biomedical Engineering, Johns Hopkins University, Baltimore, MD 21205, USA.
GRINTECH GmbH, Jena 07745, Germany.
Light Sci Appl. 2017;6(11):e17082-. doi: 10.1038/lsa.2017.82. Epub 2017 Nov 3.
This manuscript reports on the first two-photon, label-free, metabolic imaging of biological tissues at histological resolution on an extremely compact, fiber-optic endomicroscopy platform. This system provides new opportunities for performing non-invasive and functional histological imaging of internal organs , and in real time. As a routine clinical procedure, traditional histology has made significant impacts on medicine. However, the procedure is invasive and time consuming, suffers random sampling errors, and cannot provide functional information. The technology reported here features an extremely compact and flexible fiber-optic probe ~2 mm in diameter, enabling direct access to internal organs. Unprecedented two-photon imaging quality comparable to a large bench-top laser scanning microscope was achieved through technological innovations in double-clad fiber optics and miniature objective lenses (among many others). In addition to real-time label-free visualization of biological tissues with subcellular histological detail, we demonstrated for the first time two-photon endomicroscopic metabolic imaging on a functioning mouse kidney model. Such breakthroughs in nonlinear endoscopic imaging capability present numerous promising opportunities for paradigm-shifting applications in both clinical diagnosis and basic research.
本手稿报道了在一个极其紧凑的光纤内镜平台上，以组织学分辨率对生物组织进行首次双光子、无标记代谢成像。该系统为实时对内部器官进行非侵入性和功能性组织学成像提供了新机会。作为一种常规临床程序，传统组织学对医学产生了重大影响。然而，该程序具有侵入性且耗时，存在随机抽样误差，并且无法提供功能信息。这里报道的技术具有一个直径约2毫米的极其紧凑且灵活的光纤探头，能够直接进入内部器官。通过双包层光纤光学和微型物镜（以及许多其他方面）的技术创新，实现了与大型台式激光扫描显微镜相当的前所未有的双光子成像质量。除了对生物组织进行具有亚细胞组织学细节的实时无标记可视化之外，我们首次在一个功能正常的小鼠肾脏模型上展示了双光子内镜代谢成像。非线性内镜成像能力的此类突破为临床诊断和基础研究中的范式转变应用带来了众多有前景的机会。
