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猪轮状病毒样病毒（B组轮状病毒）及其抗体的体外检测

In vitro detection of porcine rotavirus-like virus (group B rotavirus) and its antibody.

作者信息

Theil K W, Saif L J

出版信息

J Clin Microbiol. 1985 May;21(5):844-6. doi: 10.1128/jcm.21.5.844-846.1985.

DOI:10.1128/jcm.21.5.844-846.1985
PMID:2987303
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC271796/
Abstract

Four isolates of porcine rotavirus-like virus (PRVLV) infected MA104 cells and induced syncytium formation after low-speed centrifugation of the inoculum onto the monolayer. Ten of 44 (23%) Ohio swine sera had PRVLV antibodies when tested by indirect immunofluorescence, using PRVLV-infected MA104 cell monolayers as antigen.

摘要

四株猪轮状病毒样病毒（PRVLV）感染了MA104细胞，并在将接种物低速离心到单层细胞上后诱导了合胞体形成。当以感染PRVLV的MA104细胞单层为抗原，通过间接免疫荧光法检测时，44份俄亥俄州猪血清中有10份（23%）含有PRVLV抗体。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/760b/271796/197caeebcf51/jcm00118-0207-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/760b/271796/dcb5efc278f7/jcm00118-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/760b/271796/197caeebcf51/jcm00118-0207-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/760b/271796/dcb5efc278f7/jcm00118-0207-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/760b/271796/197caeebcf51/jcm00118-0207-b.jpg

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本文引用的文献

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Arch Virol. 1984;80(4):291-303. doi: 10.1007/BF01311220.
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Comparison of atypical rotaviruses from calves, piglets, lambs and man.来自犊牛、仔猪、羔羊和人类的非典型轮状病毒的比较。
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Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses.猪轮状病毒的分离、血清分型及与其他轮状病毒的抗原性比较。
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Physicochemical characterization of porcine pararotavirus and detection of virus and viral antibodies using cell culture immunofluorescence.猪副轮状病毒的理化特性及利用细胞培养免疫荧光法检测病毒和病毒抗体
J Clin Microbiol. 1987 Feb;25(2):268-72. doi: 10.1128/jcm.25.2.268-272.1987.
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The rhesus rotavirus gene encoding protein VP3: location of amino acids involved in homologous and heterologous rotavirus neutralization and identification of a putative fusion region.恒河猴轮状病毒编码蛋白VP3的基因：参与同源和异源轮状病毒中和作用的氨基酸位置及一个假定融合区域的鉴定
Proc Natl Acad Sci U S A. 1988 Feb;85(3):645-9. doi: 10.1073/pnas.85.3.645.
7
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8
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