Pujol J P, Penfornis H, Arenzana-Seisdedos F, Bocquet J, Farjanel J, Rattner A, Brisset M, Virelizier J L, Beliard R, Loyau G
Exp Cell Res. 1985 May;158(1):63-74. doi: 10.1016/0014-4827(85)90431-8.
Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-mediated. The active material, of apparent molecular weight 12 000-20 000, also displays the properties of the mononuclear cell factor (MCF) previously described by others, since its stimulates collagenase and PGE2 release by the cultured synovial cells. Furthermore, it co-purifies with interleukin 1 (IL 1) as shown by lymphocyte-activating factor activity. This strongly suggests that IL 1 could be responsible for some (or all) the effects observed on MCF-exposed synovial cells. From these data, we deduce the possibility that mononuclear cells may participate in limiting synovial collagen deposition in rheumatoid arthritis.
人PHA刺激的单核细胞产生一种因子，该因子抑制滑膜细胞胶原蛋白和非胶原蛋白的合成，而增强透明质酸（HA）的产生。环氧化酶抑制剂吲哚美辛（10⁻⁴ - 10⁻⁶ M）可抑制这种作用，提示其机制是由前列腺素介导的。这种表观分子量为12000 - 20000的活性物质，也表现出其他人先前描述的单核细胞因子（MCF）的特性，因为它能刺激培养的滑膜细胞释放胶原酶和前列腺素E2。此外，如淋巴细胞激活因子活性所示，它与白细胞介素1（IL - 1）共纯化。这强烈提示IL - 1可能是观察到的对暴露于MCF的滑膜细胞产生的某些（或全部）效应的原因。从这些数据中，我们推断单核细胞可能参与限制类风湿关节炎中滑膜胶原沉积的可能性。
