Rizzino A, Bowen-Pope D F
Dev Biol. 1985 Jul;110(1):15-22. doi: 10.1016/0012-1606(85)90058-2.
In this report, we demonstrate that F9 and PC-13 embryonal carcinoma (EC) cells do not bind significant amounts of platelet-derived growth factor (PDGF), whereas the endoderm-like differentiated cells derived from EC cells do. The F9-differentiated cells exhibit approximately 8300 receptors per cell, with an apparent dissociation constant of 30 pM. Two endoderm-like cell lines, PSA-5E and PYS-2, also bind PDGF and exhibit approximately 4800 and 23,500 receptors per cell, respectively. The lack of PDGF binding by the parental EC cells is consistent with their release of a factor(s) that is closely related to PDGF. This factor(s) competes with PDGF for binding to membrane receptors and is recognized by antibodies raised against PDGF. However, this factor(s) does not appear to be antigenically identical to PDGF. We also show that production of this PDGF-like factor(s) is reduced more than 90% when F9 EC cells differentiate into cells that bind PDGF. Thus, our findings indicate that EC cells release a factor(s) that should be capable of binding to their differentiated cells. This raises the possibility that PDGF, or a closely related factor, can influence cell proliferation and/or cell behavior of early embryonic cells.
在本报告中，我们证明F9和PC - 13胚胎癌细胞不会结合大量的血小板衍生生长因子（PDGF），而源自胚胎癌细胞的内胚层样分化细胞则会结合。F9分化细胞每个细胞表现出约8300个受体，表观解离常数为30 pM。两种内胚层样细胞系PSA - 5E和PYS - 2也结合PDGF，每个细胞分别表现出约4800个和23500个受体。亲代胚胎癌细胞缺乏PDGF结合与其释放一种与PDGF密切相关的因子一致。这种因子与PDGF竞争结合膜受体，并被针对PDGF产生的抗体识别。然而，这种因子在抗原性上似乎与PDGF并不相同。我们还表明，当F9胚胎癌细胞分化为结合PDGF的细胞时，这种类PDGF因子的产生减少了90%以上。因此，我们的研究结果表明胚胎癌细胞释放一种应该能够与其分化细胞结合的因子。这增加了PDGF或密切相关因子能够影响早期胚胎细胞增殖和/或细胞行为的可能性。
