Laboratory for Molecular Reproduction and Genetics, Department of Anatomy, All India Institute of Medical Sciences, New Delhi 29, India.
J Assist Reprod Genet. 2012 Sep;29(9):861-7. doi: 10.1007/s10815-012-9801-3. Epub 2012 Jun 13.
Standard semen parameters are poor predictors of fertility potential. To date, apart from, paternal karyotyping sperm factors are not evaluated in recurrent pregnancy loss (RPL), only recent studies have emphasized the role of sperm factors in early embryonic development as sperm transcribes genes critical for early embryonic development. Sperm DNA integrity is useful diagnostic and prognostic marker and has clinical implications in idiopathic recurrent pregnancy loss (iRPL) following spontaneous conception. The aim of this study was to assess DNA integrity in cases experiencing iRPL following spontaneous conception.
Semen samples from 45 patients and 20 controls were analyzed as per WHO 1999 guidelines and sperm chromatin structure assay (SCSA) was used to measure DNA fragmentation index (DFI).
By applying receiver operating curve (ROC) analysis, sperm DFI of approximately 26 % was found in male partner of couples experiencing iRPL.
Our data indicate that sperm from men with a history of iRPL have a higher percentage of DNA damage as compared to control group, and this can explain pregnancy loss in these patients. Men with higher DFI are infertile whereas men with lower DFI (26 %) are able to conceive but experience recurrent pregnancy loss. Thus it is important to evaluate sperm DFI in couples experiencing iRPL to understand exact aetiology of RPL and determine prognosis and management.
标准精液参数是预测生育潜能的不良指标。迄今为止，除了父系染色体核型分析外，反复妊娠丢失（RPL）中并未评估精子因素，只有最近的研究强调了精子因素在早期胚胎发育中的作用，因为精子转录了对早期胚胎发育至关重要的基因。精子 DNA 完整性是一种有用的诊断和预后标志物，在自发性妊娠后特发性复发性妊娠丢失（iRPL）中具有临床意义。本研究旨在评估自发性妊娠后发生 iRPL 的情况下 DNA 完整性。
根据世界卫生组织 1999 年的指南分析了 45 例患者和 20 例对照的精液样本，并使用精子染色质结构分析（SCSA）来测量 DNA 碎片化指数（DFI）。
通过应用接收器工作特征曲线（ROC）分析，发现经历 iRPL 的夫妇中男性伴侣的精子 DFI 约为 26%。
我们的数据表明，与对照组相比，有 iRPL 病史的男性精子的 DNA 损伤百分比更高，这可以解释这些患者的妊娠丢失。DFI 较高的男性不育，而 DFI 较低（26%）的男性能够受孕但经历反复妊娠丢失。因此，在经历 iRPL 的夫妇中评估精子 DFI 以了解 RPL 的确切病因并确定预后和管理非常重要。
