精液参数异常男性精子DNA碎片化与血清脂质谱的相关性：一项分析性横断面研究。
The Association of Sperm DNA Fragmentation With Serum Lipid Profile Among Males With Abnormal Semen Parameters: An Analytical Cross-Sectional Study.
作者信息
Thankamoni Raju Kumaran, Tamgire Dharmaraj W, Dasari Papa, Adole Prashant S, Jinkala Sreerekha, Ns Kubera, Murugiah Viveka
机构信息
Anatomy, Velammal Medical College Hospital and Research Institute, Madurai, IND.
Anatomy, Jawaharlal Institute of Postgraduate Medical Education and Research, Puducherry, IND.
出版信息
Cureus. 2024 May 13;16(5):e60243. doi: 10.7759/cureus.60243. eCollection 2024 May.
BACKGROUND
Through the ages, infertility, affecting 8% to 12% of couples worldwide, has been a perturbing clinical problem. Approximately 40% to 50% of all infertility cases are due to 'male factor' infertility. Semen analysis is crucial in routinely evaluating idiopathic male infertility. Studies support the idea that semen parameters are associated with serum lipids and sperm DNA fragmentation (SDF). Therefore, it is possible to evaluate male infertility by serum lipid levels, especially before assisted reproduction technology, and modify it by bringing about lifestyle modifications. This study aimed to measure the correlation of SDF with levels of total cholesterol (TC), triglycerides (TG), very low-density lipoprotein (VLDL), low-density lipoprotein (LDL), and high-density lipoprotein (HDL) among males with abnormal semen parameters.
METHODS
A cross-sectional analytical study was conducted in the infertility clinic of a tertiary care hospital. A total of 106 infertile males with abnormal semen analysis as per the WHO criteria (2010) were enrolled in the study. After routine semen analysis, SDF was studied using the comet assay. The serum fasting lipid profile was analyzed using the spectrophotometric kit in the autoanalyzer. The relationship of SDF with serum lipid profile parameters was analyzed.
RESULTS
Out of 106 infertile men, 52% (n = 55) had severe SDF. A modest positive correlation was observed between SDF (percentage of DNA in comet tail) and serum lipid values (serum TG, serum LDL, and serum VLDL).
CONCLUSIONS
Our study is novel in its research on the correlation between SDF and serum lipid values. Based on the findings of our study, it can be concluded that a significant level of SDF was observed in men with high levels of serum TG, LDL, and VLDL. This provokes a potential relationship between sperm DNA integrity and serum lipid profile, which warrants further research.
背景
长久以来，不孕症一直是一个令人困扰的临床问题，全球约8%至12%的夫妇受其影响。所有不孕症病例中，约40%至50%归因于“男性因素”不孕。精液分析对于常规评估特发性男性不育至关重要。研究支持精液参数与血脂及精子DNA碎片化（SDF）相关的观点。因此，通过血脂水平评估男性不育是可行的，尤其是在辅助生殖技术之前，并可通过改变生活方式加以改善。本研究旨在测量精液参数异常男性中SDF与总胆固醇（TC）、甘油三酯（TG）、极低密度脂蛋白（VLDL）、低密度脂蛋白（LDL）和高密度脂蛋白（HDL）水平之间的相关性。
方法
在一家三级护理医院的不孕不育诊所进行了一项横断面分析研究。根据世界卫生组织（2010年）标准，共纳入106名精液分析异常的不育男性。进行常规精液分析后，采用彗星试验研究SDF。使用自动分析仪中的分光光度试剂盒分析血清空腹血脂谱。分析SDF与血清血脂谱参数之间的关系。
结果
106名不育男性中，52%（n = 55）有严重的SDF。观察到SDF（彗星尾中的DNA百分比）与血脂值（血清TG、血清LDL和血清VLDL）之间存在适度的正相关。
结论
我们的研究在SDF与血脂值相关性的研究方面具有创新性。根据我们的研究结果，可以得出结论，血清TG、LDL和VLDL水平高的男性中观察到了显著水平的SDF。这引发了精子DNA完整性与血清血脂谱之间的潜在关系，值得进一步研究。
Zotero 插件