从阿尔茨海默病和其他神经系统疾病患者采集的血浆和脑脊液中无肌动蛋白的Gc球蛋白浓度变化的病理生理意义。

Pathophysiological implications of actin-free Gc-globulin concentration changes in blood plasma and cerebrospinal fluid collected from patients with Alzheimer's disease and other neurological disorders.

作者信息

Kułakowska Alina, Tarasiuk Joanna, Kapica-Topczewska Katarzyna, Chorąży Monika, Pogorzelski Robert, Kulczyńska-Przybik Agnieszka, Mroczko Barbara, Bucki Robert

机构信息

Department of Neurology, Medical University of Białystok, Poland.

Department of Neurodegeneration Diagnostics, Medical University of Białystok, Poland.

出版信息

Adv Clin Exp Med. 2018 Aug;27(8):1075-1080. doi: 10.17219/acem/70441.

DOI:10.17219/acem/70441

PMID:29905407

Abstract

BACKGROUND

The extracellular actin scavenging system (EASS) is composed of plasma Gc-globulin and gelsolin, and is responsible for the elimination of toxic actin from the bloodstream.

OBJECTIVES

In this study, we assessed the actin-free Gc-globulin concentrations in blood plasma and cerebrospinal fluid (CSF) obtained from subjects with neurodegenerative and inflammatory diseases of the central nervous system (CNS) as well as in a control group.

MATERIAL AND METHODS

Using an enzyme-linked immunosorbent assay (ELISA), we measured the actinfree Gc-globulin concentrations in blood plasma and CSF obtained from subjects diagnosed with Alzheimer's disease (AD) (n = 20), amyotrophic lateral sclerosis (ALS) (n = 12), multiple sclerosis (MS) (n = 42), tick-borne encephalitis (TBE) (n = 12), and from a control group (n = 20).

RESULTS

The concentrations of free Gc-globulin in plasma collected from patients diagnosed with AD (509.6 ±87.6 mg/L) and ALS (455.5 ±99.8 mg/L) did not differ significantly between each other, but were significantly higher compared to the reference group (311.7 ±87.5 mg/L) (p < 0.001 and p < 0.006, respectively) as well as to MS (310.8 ±66.6 mg/L) (p < 0.001 and p < 0.001, respectively) and TBE (256.7 ±76 mg/L) (p < 0.001 and p < 0.003, respectively). In CSF collected from patients diagnosed with AD and ALS, the concentrations of free Gc-globulin were 2.6 ±1.1 mg/L and 2.7 ±1.9 mg/L, respectively. They did not differ significantly between each other and were significantly higher compared to the reference group (1.5 ±0.9 mg/L) (p < 0.005 and p < 0.041, respectively). Interestingly, in patients with AD, significantly higher values of Gcglobulin were detected compared to multiple sclerosis patients (1.7 ±0.9 mg/L) (p < 0.013).

CONCLUSIONS

Higher concentrations of free Gc-globulin in blood plasma and CSF collected from patients suffering from neurodegenerative diseases may indicate a potential role of this protein in their pathogenesis, and represent a potential tool for the diagnosis of CNS diseases.

摘要

背景

细胞外肌动蛋白清除系统（EASS）由血浆Gc球蛋白和凝溶胶蛋白组成，负责清除血液中的有毒肌动蛋白。

目的

在本研究中，我们评估了从患有中枢神经系统（CNS）神经退行性疾病和炎症性疾病的受试者以及对照组获得的血浆和脑脊液（CSF）中无肌动蛋白的Gc球蛋白浓度。

材料与方法

使用酶联免疫吸附测定（ELISA），我们测量了从诊断为阿尔茨海默病（AD）（n = 20）、肌萎缩侧索硬化症（ALS）（n = 12）、多发性硬化症（MS）（n = 42）、蜱传脑炎（TBE）（n = 12）的受试者以及对照组（n = 20）获得的血浆和CSF中无肌动蛋白的Gc球蛋白浓度。

结果

从诊断为AD（509.6±87.6 mg/L）和ALS（455.5±99.8 mg/L）的患者收集的血浆中，游离Gc球蛋白浓度彼此之间无显著差异，但与参考组（311.7±87.5 mg/L）相比显著更高（分别为p < 0.001和p < 0.006），与MS（310.8±66.6 mg/L）相比也显著更高（分别为p < 0.001和p < 0.001），与TBE（256.7±76 mg/L）相比同样显著更高（分别为p < 0.001和p < 0.003）。在从诊断为AD和ALS的患者收集的CSF中，游离Gc球蛋白浓度分别为2.6±1.1 mg/L和2.7±1.9 mg/L。它们彼此之间无显著差异，且与参考组（1.5±0.9 mg/L）相比显著更高（分别为p < 0.005和p < 0.041）。有趣的是，与多发性硬化症患者（1.7±0.9 mg/L）相比，AD患者中检测到的Gc球蛋白值显著更高（p < 0.013）。