大肠杆菌dgsA基因的克隆与定位
Cloning and location of the dgsA gene of Escherichia coli.
作者信息
Morris P W, Binkley J P, Henson J M, Kuempel P L
出版信息
J Bacteriol. 1985 Aug;163(2):785-6. doi: 10.1128/jb.163.2.785-786.1985.
Abstract
The dgsA locus of Escherichia coli was isolated on plasmids obtained from the library of L. Clarke and J. Carbon (Cell 9:91-99, 1976). Restriction fragment analysis and further subcloning demonstrated that the gene is located at kilobase 425 on the Bouché physical map of the terminus region (J. P. Bouché, J. Mol. Biol., 154:1-20, 1982). This corresponds to 35.2 min on the Bachmann genetic map (B. J. Bachmann, Microbiol. Rev. 47:180-230, 1983).
摘要
大肠杆菌的dgsA基因座是从L. 克拉克和J. 卡尔文文库（《细胞》9:91 - 99, 1976年）获得的质粒上分离出来的。限制性片段分析和进一步的亚克隆表明，该基因位于末端区域的布歇物理图谱上的425千碱基处（J. P. 布歇，《分子生物学杂志》，154:1 - 20, 1982年）。这相当于巴赫曼遗传图谱上的35.2分钟（B. J. 巴赫曼，《微生物学评论》47:180 - 230, 1983年）。
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