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用于检测甲型肝炎病毒特异性血清免疫球蛋白的间接免疫荧光测定法。

Indirect immunofluorescence assay for the detection of hepatitis A virus-specific serum immunoglobulins.

作者信息

Anderson D A, Coulepis A G, Chenoweth M P, Gust I D

出版信息

J Clin Microbiol. 1986 Jul;24(1):163-5. doi: 10.1128/jcm.24.1.163-165.1986.

DOI:10.1128/jcm.24.1.163-165.1986
PMID:3013929
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC268859/
Abstract

Hepatitis A virus-specific BSC-1 cells were used for the detection of serum immunoglobulins to hepatitis A virus by indirect immunofluorescence. Of 150 serum samples tested, specific immunoglobulin M was detected only in patients with serologically confirmed acute hepatitis A, while specific immunoglobulin G was detected in patients with acute or past clinical hepatitis A as well as many patients with no known history of hepatitis.

摘要

甲型肝炎病毒特异性BSC - 1细胞用于通过间接免疫荧光法检测血清中针对甲型肝炎病毒的免疫球蛋白。在检测的150份血清样本中，仅在血清学确诊为急性甲型肝炎的患者中检测到特异性免疫球蛋白M，而在急性或既往临床诊断为甲型肝炎的患者以及许多无已知肝炎病史的患者中检测到特异性免疫球蛋白G。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c342/268859/3d6d62657420/jcm00097-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c342/268859/3d6d62657420/jcm00097-0182-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/c342/268859/3d6d62657420/jcm00097-0182-a.jpg

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Indirect immunofluorescence assay for the detection of hepatitis A virus-specific serum immunoglobulins.用于检测甲型肝炎病毒特异性血清免疫球蛋白的间接免疫荧光测定法。
J Clin Microbiol. 1986 Jul;24(1):163-5. doi: 10.1128/jcm.24.1.163-165.1986.
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Serodiagnosis of viral hepatitis A: rise in antibody titre and evaluation of three methods for detecting early and late antibodies.甲型病毒性肝炎的血清学诊断：抗体滴度的升高及三种检测早期和晚期抗体方法的评估
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J Clin Microbiol. 1985 Jul;22(1):119-24. doi: 10.1128/jcm.22.1.119-124.1985.

本文引用的文献

1
Effect of hepatitis A virus infection on cell metabolism in vitro.
Proc Soc Exp Biol Med. 1984 Jan;175(1):10-5. doi: 10.3181/00379727-175-41757.
2
Persistent infection of human fibroblasts by hepatitis A virus.甲型肝炎病毒对人成纤维细胞的持续感染。
J Gen Virol. 1984 Mar;65 ( Pt 3):609-15. doi: 10.1099/0022-1317-65-3-609.
3
Monoclonal antibodies against hepatitis A virus.抗甲型肝炎病毒单克隆抗体。
J Clin Microbiol. 1983 Nov;18(5):1237-43. doi: 10.1128/jcm.18.5.1237-1243.1983.
4
Radioimmunofocus assay for quantitation of hepatitis A virus in cell cultures.用于定量细胞培养物中甲型肝炎病毒的放射免疫聚焦测定法。
J Clin Microbiol. 1983 May;17(5):834-9. doi: 10.1128/jcm.17.5.834-839.1983.
5
Detection of hepatitis A virus and antibody by solid-phase radioimmunoassay and enzyme-linked immunosorbent assay with monoclonal antibodies.用单克隆抗体通过固相放射免疫测定法和酶联免疫吸附测定法检测甲型肝炎病毒及抗体。
J Clin Microbiol. 1985 Jul;22(1):119-24. doi: 10.1128/jcm.22.1.119-124.1985.
6
The origin of the HM175 strain of hepatitis A virus.甲型肝炎病毒HM175毒株的起源。
J Infect Dis. 1985 Feb;151(2):365-7. doi: 10.1093/infdis/151.2.365.
7
Solid-phase enzyme-linked immunosorbent assay for detection of hepatitis A-specific immunoglobulin M.用于检测甲型肝炎特异性免疫球蛋白M的固相酶联免疫吸附测定法
J Clin Microbiol. 1979 Apr;9(4):459-65. doi: 10.1128/jcm.9.4.459-465.1979.
8
Propagation of human hepatitis A virus in cell culture in vitro.
Proc Soc Exp Biol Med. 1979 Feb;160(2):213-21. doi: 10.3181/00379727-160-40422.
9
Enzyme-linked immunosorbent assay for detection of hepatitis A antigen in stool and antibody to hepatitis A antigen in sera: comparison with solid-phase radioimmunoassay, immune electron microscopy, and immune adherence hemagglutination assay.酶联免疫吸附测定法检测粪便中的甲型肝炎抗原及血清中甲型肝炎抗原抗体：与固相放射免疫测定法、免疫电镜及免疫黏附血凝试验的比较
J Clin Microbiol. 1978 Feb;7(2):184-93. doi: 10.1128/jcm.7.2.184-193.1978.
10
A microtiter solid-phase radioimmunoassay for hepatitis A antigen and antibody.一种用于检测甲型肝炎抗原和抗体的微量滴定板固相放射免疫测定法。
J Immunol. 1976 Feb;116(2):349-56.