Dán Á, Rónai Z, Széll Z, Sréter T
Laboratory of Molecular Biology, Directorate of Veterinary Diagnostics, National Food Chain Safety Office, Tábornok utca 2, Budapest, H-1143, Hungary.
Laboratory of Parasitology, Fish and Bee Diseases, Directorate of Veterinary Diagnostics, National Food Chain Safety Office, Tábornok utca 2, Budapest, H-1143, Hungary.
Parasitol Res. 2018 Sep;117(9):3019-3022. doi: 10.1007/s00436-018-5977-5. Epub 2018 Jun 22.
A study was conducted to investigate genetic diversity of Echinococcus isolates collected in Hungarian slaughterhouses between 2015 and 2018. Organs of 219 animals with suspected hydatidosis were collected during routine meat inspection and sent to our laboratory. Echinococcus infection was confirmed in 65 animals. These results indicate that prevalence data based on reporting of slaughterhouses are not reliable without the appropriate training of the people responsible for meat inspection. The genetic diversity was evaluated by the DNA sequence analysis of the cytochrome c oxydase subunit 1 (cox1) mitochondrial gene. Echinococcus intermedius (n = 31), Echinococcus granulosus s.s. (n = 2), and Echinococcus multilocularis (n = 3) was identified in swine. In cattle, only E. granulosus s.s. (n = 20) was detected. E. granulosus s.s. (n = 7) was the dominant species in sheep; nevertheless, E. intermedius was also identified in two animals. E. granulosus s.s. (n = 29) and E. intermedius (n = 33) were classified in 13 and three haplotypes, respectively. The genetic diversity and haplotype network of E. granulosus s.s. were similar to that observed in some other countries of Eastern Europe. The genetic diversity of E. intermedius was low with a single dominant haplotype. Cysts were fertile in nine sheep (100%), 22 swine (61%), and three cattle (15%) indicating that all three species play a role in some extent in the epidemiology of cystic echinococcosis in Hungary. Based on the number of animals killed in the slaughterhouses involved in the present study, the rate of infection was 0.013% in sheep, 0.007% in cattle, and 0.001% in swine. As animals with hydatidosis originated from family farms, control programs should mainly focus on these facilities.
开展了一项研究，以调查2015年至2018年期间在匈牙利屠宰场收集的棘球绦虫分离株的遗传多样性。在常规肉类检查期间，收集了219只疑似患有包虫病动物的器官，并送至我们实验室。在65只动物中确诊感染了棘球绦虫。这些结果表明，在没有对负责肉类检查的人员进行适当培训的情况下，基于屠宰场报告的患病率数据不可靠。通过对细胞色素c氧化酶亚基1（cox1）线粒体基因进行DNA序列分析来评估遗传多样性。在猪中鉴定出中间棘球绦虫（n = 31）、细粒棘球绦虫原绦虫（n = 2）和多房棘球绦虫（n = 3）。在牛中，仅检测到细粒棘球绦虫原绦虫（n = 20）。细粒棘球绦虫原绦虫（n = 7）是绵羊中的优势种；不过，在两只动物中也鉴定出了中间棘球绦虫。细粒棘球绦虫原绦虫（n = 29）和中间棘球绦虫（n = 33）分别被分类为13个和3个单倍型。细粒棘球绦虫原绦虫的遗传多样性和单倍型网络与在东欧其他一些国家观察到的相似。中间棘球绦虫的遗传多样性较低，有一个单一的优势单倍型。囊肿在9只绵羊（100%）、22头猪（61%）和3头牛（15%）中具有繁殖力，这表明这三种物种在匈牙利囊型包虫病的流行病学中都在一定程度上发挥了作用。根据本研究涉及的屠宰场宰杀的动物数量，绵羊的感染率为0.013%，牛为0.007%，猪为0.001%。由于患有包虫病的动物来自家庭农场，控制计划应主要针对这些场所。
