通过分析SA11轮状病毒的抗原变体证明一个免疫显性中和位点
Demonstration of an immunodominant neutralization site by analysis of antigenic variants of SA11 rotavirus.
作者信息
Lazdins I, Sonza S, Dyall-Smith M L, Coulson B S, Holmes I H
出版信息
J Virol. 1985 Oct;56(1):317-9. doi: 10.1128/JVI.56.1.317-319.1985.
Abstract
Serotype-specific monoclonal antibodies were used to select mutants of SA11 rotavirus that were resistant to neutralization. The antigenic characteristics of these mutants were studied with with a panel of monoclonal antibodies. We isolated one type of mutant which showed a dramatic increase (greater than 10-fold) in resistance to neutralization by hyperimmune antiserum, and this together with other data indicates the presence on the rotavirus major outer shell glycoprotein of an immunodominant antigenic site involved in virus neutralization. The mutants were also useful in classifying neutralizing monoclonal antibodies.
摘要
血清型特异性单克隆抗体被用于筛选对中和作用具有抗性的SA11轮状病毒突变体。用一组单克隆抗体研究了这些突变体的抗原特性。我们分离出一种突变体，其对超免疫抗血清中和作用的抗性显著增加（超过10倍），这与其他数据一起表明轮状病毒主要外壳糖蛋白上存在一个参与病毒中和的免疫显性抗原位点。这些突变体在中和性单克隆抗体的分类中也很有用。
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