鹿乳头瘤病毒的分子克隆与核苷酸序列
Molecular cloning and nucleotide sequence of deer papillomavirus.
作者信息
Groff D E, Lancaster W D
出版信息
J Virol. 1985 Oct;56(1):85-91. doi: 10.1128/JVI.56.1.85-91.1985.
Abstract
The genome of deer papillomavirus (DPV) isolated from American white-tailed deer was cloned into pBR322, and the entire nucleotide sequence of 8,374 base pairs was determined. The overall genetic organization of the DPV genome was similar to that of other papillomaviruses. All significant open reading frames were located on one strand, and the locations of putative promoters and polyadenylation signals were similar to those identified in the closely related bovine papillomavirus type 1 (BPV-1) genome. The DPV genome was approximately colinear with BPV-1 except for a noncoding region separating the early and late regions. The regions of highest nucleotide sequence homology between DPV and BPV-1 were found in the E1 open reading frame coding for BPV-1 DNA replication function and in the L1 open reading frame, which encodes the major capsid protein of BPV-1.
摘要
从美国白尾鹿分离出的鹿乳头瘤病毒（DPV）基因组被克隆到pBR322中，并测定了8374个碱基对的完整核苷酸序列。DPV基因组的总体遗传结构与其他乳头瘤病毒相似。所有重要的开放阅读框都位于一条链上，推定启动子和多聚腺苷酸化信号的位置与在密切相关的1型牛乳头瘤病毒（BPV-1）基因组中确定的位置相似。除了分隔早期和晚期区域的非编码区外，DPV基因组与BPV-1大致共线。DPV和BPV-1之间核苷酸序列同源性最高的区域存在于编码BPV-1 DNA复制功能的E1开放阅读框以及编码BPV-1主要衣壳蛋白的L1开放阅读框中。
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