Department of Urology, Zhejiang Provincial People's Hospital, Hangzhou Medical College, 158 Shangtang Road, Hangzhou City, Zhejiang province, 310014, China.
Department of Urology, Zhejiang Provincial People's Hospital, Hangzhou Medical College, 158 Shangtang Road, Hangzhou City, Zhejiang province, 310014, China.
Biochem Biophys Res Commun. 2018 Sep 18;503(4):2459-2465. doi: 10.1016/j.bbrc.2018.06.176. Epub 2018 Jul 6.
Prostate cancer has become one of commonest urologic tumors in male. In recent years, miRNAs are continually attracting attentions of researchers for their special regulatory function in human cancers. Previous study has revealed that miR-410 acts as a biomarker for the diagnosis of prostate cancer. Whereas, the specific biological function of miR-410-3p in prostate cancer remains unknown. The aim of this study is to explore the function and molecular mechanism of miR-410-3p in prostate cancer. The high expression of miR-410-3p was examined in prostate cancer tissues and cell lines by qRT-PCR. Next, the prognostic value was identified by Kaplan Meier method. High expression of miR-410-3p indicated poor prognosis of prostate cancer patients. To investigate the biological function of miR-410-3p in prostate cancer, loss-of function assays were designed and performed in two prostate cancer cell lines (PC3 and DU145). As a result, downregulated miR-410-3p suppressed cell proliferation, migration and EMT progress. Moreover, flow cytometry analysis was performed to determine that the acceleration effects of miR-410-3p on cell apoptosis. Mechanistically, further analysis demonstrated that the effects of miR-410-3p exert oncogenic functions through downregulating PTEN. All findings in this study revealed that miR-410-3p inhibits prostate cancer progression via downregulating PTEN/AKT/mTOR signaling pathway.
前列腺癌已成为男性最常见的泌尿系肿瘤之一。近年来，miRNA 因其在人类癌症中特殊的调节功能而不断引起研究人员的关注。先前的研究表明，miR-410 可作为前列腺癌诊断的生物标志物。然而，miR-410-3p 在前列腺癌中的具体生物学功能尚不清楚。本研究旨在探讨 miR-410-3p 在前列腺癌中的功能和分子机制。通过 qRT-PCR 检测前列腺癌组织和细胞系中 miR-410-3p 的高表达。接下来，通过 Kaplan-Meier 方法确定其预后价值。miR-410-3p 的高表达表明前列腺癌患者的预后不良。为了研究 miR-410-3p 在前列腺癌中的生物学功能，在两种前列腺癌细胞系（PC3 和 DU145）中设计并进行了功能丧失实验。结果表明，下调 miR-410-3p 抑制了细胞增殖、迁移和 EMT 进展。此外，通过流式细胞术分析确定了 miR-410-3p 对细胞凋亡的加速作用。通过进一步分析发现，miR-410-3p 通过下调 PTEN 发挥致癌作用。本研究的所有发现表明，miR-410-3p 通过下调 PTEN/AKT/mTOR 信号通路抑制前列腺癌的进展。
