Saratov State University, Saratov, 410012, Russia.
Biomater Sci. 2018 Jul 24;6(8):2219-2229. doi: 10.1039/c8bm00479j.
Targeted cell delivery via magnetically sensitive microcapsules of an applied magnetic field would advance localized cell transplantation therapy, by which healthy cells can be introduced into tissues to repair damaged or diseased organs. In the present research, we implement magnetically sensitive cells via an uptake of microcapsules containing magnetic nanoparticles in their walls. As is shown in an example of the MA-104 cell line, the magnetic polyelectrolyte multilayer capsules have no toxicity effect on the cells after internalization. Microscopy methods have been used to evaluate the uptake of capsules by the cells. Magnetically sensitive cells are retained in the capillary flow when the magnetic gradient field is applied (<200 T m-1), but they proliferate at the site of retention for several days after the magnet is removed. As an example of cell manipulation, we have demonstrated a novel methodology for cell sheet isolation and transfer using cells impregnated with magnetic microcapsules. A weak enzyme treatment is used to facilitate tissue engineering assemblies by cell monolayer deposition. This type of cell monolayer assembly has provided a 3D tissue engineering construction using an externally applied magnetic field, which is modelled in this study. The approach presented in this work opens perspectives for preclinical studies of tissue and organ repair.
通过施加磁场的磁性敏感微胶囊实现靶向细胞递药，将有助于局部细胞移植治疗的发展。通过这种方法，可以将健康细胞引入组织中，以修复受损或患病的器官。在本研究中，我们通过摄取含有磁性纳米粒子的微胶囊使磁性细胞得以实现。如 MA-104 细胞系的实例所示，磁聚电解质多层胶囊在被内化后对细胞没有毒性作用。使用显微镜方法评估了细胞对胶囊的摄取。当施加磁场梯度（<200 T m-1）时，磁性敏感细胞会保留在毛细血管流中，但在磁场移除后，它们会在保留部位增殖数天。作为细胞操作的一个例子，我们展示了一种使用磁性微胶囊浸渍细胞的新颖的细胞片层分离和转移方法。弱酶处理用于通过单层细胞沉积促进组织工程组件的构建。这种类型的细胞单层组件提供了使用外部施加磁场的 3D 组织工程构建，本研究对其进行了建模。这项工作所提出的方法为组织和器官修复的临床前研究开辟了前景。
