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主要山毛榉花粉过敏原 Fag s 1 的交叉反应性和构象波动的结构基础。

Structural basis for cross-reactivity and conformation fluctuation of the major beech pollen allergen Fag s 1.

机构信息

Chemistry Department, Federal University of Minas Gerais, Belo Horizonte, Brazil.

Department of Molecular Biology, University of Salzburg, Salzburg, Austria.

出版信息

Sci Rep. 2018 Jul 12;8(1):10512. doi: 10.1038/s41598-018-28358-1.

DOI:10.1038/s41598-018-28358-1
PMID:30002383
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6043577/
Abstract

Fag s 1 is a member of the Pathogen Related protein family 10 (PR-10) and can elicit cross-reaction with IgE antibodies produced against the birch pollen allergen Bet v 1. The Nuclear Magnetic Resonance (NMR) structure of Fag s 1 is presented along with its dynamic properties. It shares 66% identity with Bet v 1 and exhibits the expected three α-helices and seven β-sheets arranged as a semi-beta barrel and exposing the residues mapped as the Bet v 1 IgE epitope. The structural dynamics of Fag s 1 were monitored on the fast and intermediate timescales, using relaxation rates. The complex dynamics of Fag s 1 are closely related to the internal cavity, and they modulate IgE and ligand binding.

摘要

山毛榉蛋白 1 是病原体相关蛋白家族 10（PR-10）的成员，能够与桦树花粉过敏原 Bet v 1 产生的 IgE 抗体发生交叉反应。本文呈现了山毛榉蛋白 1 的核磁共振（NMR）结构及其动态特性。它与 Bet v 1 具有 66%的同一性，表现出预期的三个α-螺旋和七个β-折叠，排列成半β桶，并暴露了被映射为 Bet v 1 IgE 表位的残基。使用弛豫率在快速和中间时间尺度上监测了山毛榉蛋白 1 的结构动力学。山毛榉蛋白 1 的复杂动力学与其内部空腔密切相关，并调节 IgE 和配体结合。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dba1/6043577/c8feb77256b0/41598_2018_28358_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dba1/6043577/78ed2bbb64c9/41598_2018_28358_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dba1/6043577/5d4b5fcfc6e7/41598_2018_28358_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dba1/6043577/eb1bee79104c/41598_2018_28358_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dba1/6043577/3aa88fea41c0/41598_2018_28358_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dba1/6043577/c8feb77256b0/41598_2018_28358_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dba1/6043577/78ed2bbb64c9/41598_2018_28358_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dba1/6043577/5d4b5fcfc6e7/41598_2018_28358_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dba1/6043577/eb1bee79104c/41598_2018_28358_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dba1/6043577/3aa88fea41c0/41598_2018_28358_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dba1/6043577/c8feb77256b0/41598_2018_28358_Fig5_HTML.jpg

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