Lee Sun Min, Kim Nayoung, Park Ji Hyun, Nam Ryoung Hee, Yoon Kichul, Lee Dong Ho
Department of Internal Medicine, Seoul National University Bundang Hospital, Seongnam, Korea.
Department of Internal Medicine and Liver Research Institute, Seoul National University College of Medicine, Seoul, Korea.
J Cancer Prev. 2018 Jun;23(2):70-76. doi: 10.15430/JCP.2018.23.2.70. Epub 2018 Jun 30.
Gut microbiota contributes to intestinal and immune homeostasis through host-microbiota interactions. Distribution of the gut microbiota differs according to the location in the gastrointestinal tract. Although the microbiota properties change with age, evidence for the regional difference of gut microbiota has been restricted to the young. The aim of this study is to compare the gut microbiota between terminal ileum and cecum of old rats.
We analyzed gut microbiome of luminal contents from ileum and cecum of 74-week-old and 2-year-old rats (corresponding to 60-year and 80-year-old of human age) by metagenome sequencing of 16S rRNA.
Inter-individual variation (beta diversity) of microbiota was higher in ileum than in cecum. Conversely, alpha diversity of microbiota composition was higher in cecum than in ileum. were more abundant in ileum compared to cecum while and were more enriched in cecum. The proportions of were increased in cecal microbiota of 2-year-old rats compared to 74-week-old rats.
Major regional distinctions of microbiota between ileum and cecum of old rats appear consistent with those of young rats. Age-related alterations of gut microbiota in old rats seem to occur in minor compositions.
肠道微生物群通过宿主-微生物群相互作用有助于维持肠道和免疫稳态。肠道微生物群的分布因胃肠道位置而异。尽管微生物群特性随年龄变化，但肠道微生物群区域差异的证据仅限于年轻人。本研究的目的是比较老年大鼠回肠末端和盲肠的肠道微生物群。
我们通过对16S rRNA进行宏基因组测序，分析了74周龄和2岁大鼠（相当于人类年龄的60岁和80岁）回肠和盲肠腔内容物的肠道微生物组。
回肠中微生物群的个体间差异（β多样性）高于盲肠。相反，盲肠中微生物群组成的α多样性高于回肠。与盲肠相比，回肠中 更丰富，而 和 在盲肠中更富集。与74周龄大鼠相比，2岁大鼠盲肠微生物群中 的比例增加。
老年大鼠回肠和盲肠微生物群的主要区域差异似乎与年轻大鼠一致。老年大鼠肠道微生物群与年龄相关的变化似乎发生在次要组成部分。
