West China School of Public Health, Sichuan University, Chengdu 610041, China.
West China School of Public Health, Sichuan University, Chengdu 610041, China; Chengdu Center for Disease Control and Prevention, Chengdu, Sichuan 610047, China.
Biosens Bioelectron. 2018 Oct 15;117:678-689. doi: 10.1016/j.bios.2018.06.062. Epub 2018 Jun 30.
DNA has well-defined ability to recognize a wide variety of targets, such as small biological molecules, proteins, inorganic ions and small organic molecules. As molecular recognition elements, DNA can be used to build simple, rapid and sensitive biosensors for detection of these targets. DNA-based SPR sensors are considered to be a real-time and label-free tool. We present a systematical and critical review on DNA-based SPR biosensors and their signal amplification via various strategies, focusing on recent advances in nanomaterials, novel DNA amplifications, redox reactions on surface, enzyme amplifications, as well as promising multiplex amplification strategies.
DNA 具有识别多种目标的明确能力，例如小分子生物分子、蛋白质、无机离子和小分子有机分子。作为分子识别元件，DNA 可用于构建用于检测这些目标的简单、快速和灵敏的生物传感器。基于 DNA 的 SPR 传感器被认为是一种实时和无标记的工具。我们对基于 DNA 的 SPR 生物传感器及其通过各种策略进行的信号放大进行了系统和批判性的回顾，重点介绍了纳米材料、新型 DNA 扩增、表面氧化还原反应、酶扩增以及有前途的多重扩增策略方面的最新进展。
