State Key Laboratory of Quality Research in Chinese Medicine, Institute of Chinese Medical Sciences, University of Macau, Macao SAR, China.
Choi Kai Yau College, University of Macau, Macao SAR, China.
Carbohydr Polym. 2018 Oct 1;197:9-16. doi: 10.1016/j.carbpol.2018.05.066. Epub 2018 May 23.
The polysaccharides obtained from the root of Ilex asprella, namely IAPS-1 and IAPS-2, with immunoregulatory activity were studied. Two polysaccharides were isolated and purified by Cellulose DEAE-52 and Sephadex columns. The structure of IAPS-1 was elucidated as 1,6-linked α-d-glucopyranosyl main chain with branch chain substituted at C-2 and/or C-4 position. For IAPS-2, the backbone is composed of 1, 4-linked α-d-glucose, galactose and rhamnose, and branched chains consists of arabinose, rhamnose and galacturonic acid, as confirmed by partial acid hydrolysis and NMR study. Further, the immunoregulatory activity of IAPS-1 and IAPS-2 was tested with the murine macrophages. Particularly, IAPS-2 polysaccharide can more effectively enhance the secretion of major inflammatory cytokines in macrophages, such as TNF-α, IL-1β, IL-12, compared with IAPS-1.
从冬青科苦丁茶的根中提取到具有免疫调节活性的多糖，即 IAPS-1 和 IAPS-2，并对其进行了研究。通过纤维素 DEAE-52 和葡聚糖凝胶 Sephadex 柱对两种多糖进行分离和纯化。IAPS-1 的结构被阐明为具有 1,6-连接的α-d-吡喃葡萄糖主链，在 C-2 和/或 C-4 位置取代有支链。对于 IAPS-2，其骨架由 1,4-连接的α-d-葡萄糖、半乳糖和鼠李糖组成，支链由阿拉伯糖、鼠李糖和半乳糖醛酸组成，这是通过部分酸水解和 NMR 研究证实的。此外，用小鼠巨噬细胞测试了 IAPS-1 和 IAPS-2 的免疫调节活性。特别是，与 IAPS-1 相比，IAPS-2 多糖更有效地增强了巨噬细胞中主要炎症细胞因子（如 TNF-α、IL-1β、IL-12）的分泌。
