Sanson-le Pors M J, Casin I M, Collatz E
Antimicrob Agents Chemother. 1985 Aug;28(2):315-9. doi: 10.1128/AAC.28.2.315.
Three clinical isolates of Haemophilus ducreyi, representing at least two subtypes, were shown to be resistant to streptomycin and kanamycin. They also produced a beta-lactamase and chloramphenicol acetyltransferase and were resistant to tetracycline. In the three strains the resistance to both aminoglycoside antibiotics was encoded by a plasmid of ca. 4.7 kilobases which apparently did not carry ampicillin, chloramphenicol, or tetracycline resistance genes, as determined after transfer to Escherichia coli by transformation. Resistance to streptomycin and kanamycin was due to the presence of two aminoglycoside phosphotransferases (APH). The enzyme modifying kanamycin was a 3',5"-APH of type I [APH(3',5")-I], as inferred from its substrate profile and immunological cross-reactivity with the APH(3',5")-I encoded by the transposable element Tn903. However, the APH(3',5")-I gene in H. ducreyi did not appear to be carried by Tn903.
三株临床分离的杜克雷嗜血杆菌，至少代表两种亚型，已显示对链霉素和卡那霉素耐药。它们还产生β-内酰胺酶和氯霉素乙酰转移酶，并且对四环素耐药。在这三株菌株中，对两种氨基糖苷类抗生素的耐药性由一个约4.7千碱基的质粒编码，通过转化转移到大肠杆菌后测定，该质粒显然不携带氨苄青霉素、氯霉素或四环素耐药基因。对链霉素和卡那霉素的耐药性是由于存在两种氨基糖苷磷酸转移酶（APH）。修饰卡那霉素的酶是I型3',5"-APH [APH(3',5")-I]，这是根据其底物谱以及与由转座元件Tn903编码的APH(3',5")-I的免疫交叉反应推断出来的。然而，杜克雷嗜血杆菌中的APH(3',5")-I基因似乎不是由Tn903携带的。
