Department of Biomedical Engineering, University of Michigan, Ann Arbor, MI, USA.
Department of Internal Medicine, Division of Pulmonary and Critical Care Medicine, University of Michigan Medical School, Ann Arbor, MI, USA.
Respirology. 2018 Nov;23(11):993-1003. doi: 10.1111/resp.13383. Epub 2018 Aug 13.
The proteome is the study of the protein content of a definable component of an organism in biology. However, the tissue-specific expression of proteins and the varied post-translational modifications, splice variants and protein-protein complexes that may form, make the study of protein a challenging yet vital tool in answering many of the unanswered questions in medicine and biology to date. Indeed, the spatial, temporal and functional composition of proteins in the human body has proven difficult to elucidate for many years. Given the effect of microRNA and epigenetic regulation on silencing and enhancing gene transcription, the study of protein arguably provides more accurate information on homeostasis and perturbation in health and disease. There have been significant advances in the field of proteomics in recent years, with new technologies and platforms available to the research community. In this review, we briefly discuss some of these new technologies and developments in the context of respiratory disease. We also discuss the types of data science approaches to analyses and interpretation of the large volumes of data generated in proteomic studies. We discuss the application of these technologies with regard to respiratory disease and highlight the potential for proteomics in generating major advances in the understanding of respiratory pathophysiology into the future.
蛋白质组学是生物学中对生物体中可定义成分的蛋白质含量的研究。然而，蛋白质的组织特异性表达以及可能形成的各种翻译后修饰、剪接变体和蛋白质-蛋白质复合物，使得蛋白质研究成为回答迄今为止医学和生物学中许多未解决问题的极具挑战性但至关重要的工具。事实上，多年来，人体蛋白质的空间、时间和功能组成一直难以阐明。鉴于 microRNA 和表观遗传调控对基因转录的沉默和增强的影响，蛋白质的研究可以提供关于健康和疾病中内稳态和干扰的更准确信息。近年来，蛋白质组学领域取得了重大进展，研究界有了新的技术和平台。在这篇综述中，我们简要讨论了这些新技术和新进展在呼吸疾病中的应用。我们还讨论了数据分析科学方法在分析和解释蛋白质组学研究中产生的大量数据方面的应用。我们讨论了这些技术在呼吸疾病中的应用，并强调了蛋白质组学在未来对呼吸病理生理学的理解产生重大进展的潜力。
