鼠心室心肌细胞的转录组分析。
Transcriptomic analyses of murine ventricular cardiomyocytes.
机构信息
Ion Channels and Channelopathies Laboratory, Institute for Biochemistry and Molecular Medicine, University of Bern, 3012 Bern, Switzerland.
Interfaculty Bioinformatics Unit and Swiss Institute of Bioinformatics, University of Bern, 3012 Bern, Switzerland.
出版信息
Sci Data. 2018 Aug 21;5:180170. doi: 10.1038/sdata.2018.170.
Mice are used universally as model organisms for studying heart physiology, and a plethora of genetically modified mouse models exist to study cardiac disease. Transcriptomic data for whole-heart tissue are available, but not yet for isolated ventricular cardiomyocytes. Our lab therefore collected comprehensive RNA-seq data from wildtype murine ventricular cardiomyocytes as well as from knockout models of the ion channel regulators CASK, dystrophin, and SAP97. We also elucidate ion channel expression from wild-type cells to help forward the debate about which ion channels are expressed in cardiomyocytes. Researchers studying the heart, and especially cardiac arrhythmias, may benefit from these cardiomyocyte-specific transcriptomic data to assess expression of genes of interest.
老鼠被普遍用作研究心脏生理学的模型生物，并且存在大量的基因修饰小鼠模型来研究心脏疾病。全心脏组织的转录组数据是可用的，但尚未用于分离的心室心肌细胞。因此，我们的实验室从野生型鼠心室心肌细胞以及离子通道调节剂 CASK、肌营养不良蛋白和 SAP97 的基因敲除模型中收集了全面的 RNA-seq 数据。我们还阐明了来自野生型细胞的离子通道表达情况，以帮助推进关于哪些离子通道在心肌细胞中表达的争论。研究心脏的研究人员，特别是心律失常的研究人员，可能会从这些心肌细胞特异性转录组数据中受益，以评估感兴趣基因的表达。
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