State Key Laboratory of Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China.
NanJing Foreign Language School, Nanjing, China.
Nanomedicine. 2018 Nov;14(8):2619-2631. doi: 10.1016/j.nano.2018.07.014. Epub 2018 Aug 18.
Timely detection is crucial for successful treatment of cancer. The current study describes a new approach that involves utilization of the tumor cell environment for bioimaging with in-situ biosynthesized nanoscale gold and iron probes and subsequent dissemination of Au-Fe nanoclusters from cargo exosomes within the circulatory system. We have isolated the Au-Fe cargo exosomes from the blood of the treated murine models after in situ biosyntheses from their respective pre-ionic solutions (HAuCl, FeCl), whereas NaSeO supplementation added into Au lethal effect. The microarray data of various differentially expressed genes revealed the up-regulated tumor ablation and metal binding genes in SGC-7901 cell lines after treatment with Au-Fe-Se triplet ionic solution. The isolation of Au-Fe nanoclusters cargo exosomes (nano in nano) after secretion from deeply seated tumors may help in early diagnosis and reveal the tumor ablation status during and after the relevant treatment like radio-chemo therapies et al.
及时检测对于癌症的成功治疗至关重要。本研究描述了一种新方法，该方法涉及利用肿瘤细胞环境进行生物成像，使用原位生物合成的纳米级金和铁探针，并随后在循环系统中从货物外泌体中传播 Au-Fe 纳米簇。我们已经从各自的前离子溶液（HAuCl，FeCl）中对来自处理过的鼠模型的血液进行原位生物合成后，从血液中分离出了 Au-Fe 货物外泌体，而 NaSeO 补充剂则添加到了 Au 的致死效应中。各种差异表达基因的微阵列数据显示，在用 Au-Fe-Se 三重离子溶液处理 SGC-7901 细胞系后，上调了肿瘤消融和金属结合基因。从深位肿瘤分泌后分离出的 Au-Fe 纳米簇货物外泌体（纳米中的纳米）可能有助于早期诊断，并在相关治疗（如放化疗等）期间和之后揭示肿瘤消融状态。
