Souza Clarissa P, Schissler Jennifer R, Contreras Elena T, Dow Steven W, Hopkins Leone S, Coy Jonathan W, Rosychuk Rod A W, Hawley Jennifer R, Lappin Michael R
Department of Clinical Sciences, College of Veterinary Medicine and Biomedical Sciences, Colorado State University, 300 West Drake Road, Fort Collins, CO, 80525, USA.
Vet Dermatol. 2018 Dec;29(6):482-e162. doi: 10.1111/vde.12681. Epub 2018 Aug 23.
Juvenile onset generalized demodicosis (JOGD) is thought to occur due to immunological abnormalities and is over-represented in pit bull terrier-type dogs.
Twelve pit bull terrier-type dogs with JOGD and 12 age-matched healthy pit bull terrier-type dogs.
To investigate immunological differences between age-matched healthy and JOGD pit bull terrier-type dogs by flow cytometry, multiplex, molecular and serological assays.
Flow cytometry quantified B cells expressing MHCII or surface-bound IgG, CD4+ T cells expressing MHCII, CD8 T cells expressing MHCII or CD11a, neutrophil and monocyte markers. Surface expression was quantified by calculating the geometric mean fluorescence index. The Wilcoxon rank sum test was used to compare median results for IL-2, IL-4, IL-6, IL-7, IL-8, IL-10, IL-13, IL-18, FOXP3, monocyte chemoattractant protein-1, GM-CSF, KC, IgE, IgA, IgG, IgM, C-reactive protein, lymphocyte, neutrophil and monocyte in the groups. IFN-gamma, IP-10, IL-15, IL-31 and TNF-alpha also were measured; however, insufficient dogs (<5) had values that were in range of the assay to allow for statistical evaluation. Significance was defined as P < 0.05.
Serum concentrations of IL-2, IL-18 and MCP-1 were significantly higher (P = 0.01, P = 0.01, P = 0.04) in the JOGD group. Also, IgA median value was significantly higher (P = 0.002) in pit bull terrier-type dogs with JOGD. Flow cytometry revealed that T-cell, neutrophil and monocyte markers were not different between groups.
Results suggest an appropriate compensatory immune response by pit bull terrier-type dogs in the JOGD group and do not support the explanation of global immune deficiency in these dogs.
幼年型全身性蠕形螨病（JOGD）被认为是由于免疫异常而发生的，并且在比特斗牛梗犬种中发病率过高。
12只患有JOGD的比特斗牛梗犬和12只年龄匹配的健康比特斗牛梗犬。
通过流式细胞术、多重检测、分子检测和血清学检测，研究年龄匹配的健康比特斗牛梗犬与患有JOGD的比特斗牛梗犬之间的免疫差异。
流式细胞术对表达MHCII或表面结合IgG的B细胞、表达MHCII的CD4 + T细胞、表达MHCII或CD11a的CD8 T细胞、中性粒细胞和单核细胞标志物进行定量。通过计算几何平均荧光指数对表面表达进行定量。采用Wilcoxon秩和检验比较两组中IL-2、IL-4、IL-6、IL-7、IL-8、IL-10、IL-13、IL-18、FOXP3、单核细胞趋化蛋白-1、GM-CSF、KC、IgE、IgA、IgG、IgM、C反应蛋白、淋巴细胞、中性粒细胞和单核细胞的中位数结果。还检测了IFN-γ、IP-10、IL-15、IL-31和TNF-α；然而，犬只数量不足（<5只），其检测值在检测范围内，无法进行统计评估。显著性定义为P < 0.05。
JOGD组中IL-2、IL-18和MCP-1的血清浓度显著更高（P = 0.01，P = 0.01，P = 0.04）。此外，患有JOGD的比特斗牛梗犬的IgA中位数显著更高（P = 0.002）。流式细胞术显示两组之间T细胞、中性粒细胞和单核细胞标志物无差异。
结果表明JOGD组中的比特斗牛梗犬有适当的代偿性免疫反应，不支持这些犬存在全身性免疫缺陷的解释。
