Babak Myeloma Group, Department of Pathological Physiology, Faculty of Medicine, Masaryk University, Brno, Czech Republic.
Central European Institute of Technology, Masaryk University, Brno, Czech Republic.
Hematol Oncol. 2018 Dec;36(5):786-791. doi: 10.1002/hon.2554. Epub 2018 Sep 13.
Multiple myeloma is the second most common hematological malignancy characterized by focal lesions of malignant plasma cells in the bone marrow. These lesions contain subclones that directly influence survival of patients. Bone marrow biopsies are single-site biopsies and thus cannot contain all information about the tumor. In contrast, liquid biopsies analyze circulating cells and molecules that are secreted from all sites of the tumor. Long noncoding RNA molecules are one class of these molecules. We performed a two-phase biomarker study investigating lncRNA expression profiles in exosomes of peripheral blood serum of newly diagnosed multiple myeloma (MM) patients, monoclonal gammopathy of undetermined significance (MGUS) patients in comparison with healthy donors (HD). Surprisingly, this analysis revealed dysregulation of only one exosomal lncRNA PRINS in MM vs HD. Overall, MM and MGUS patients were distinguished from HD with sensitivity of 84.9% and specificity of 83.3%. Our study suggests a possible diagnostic role for exosomal lncRNA PRINS in monoclonal gammopathies patients.
多发性骨髓瘤是第二大常见的血液系统恶性肿瘤，其特征是骨髓中恶性浆细胞的局灶性病变。这些病变包含直接影响患者生存的亚克隆。骨髓活检是单点活检，因此不能包含关于肿瘤的所有信息。相比之下，液体活检分析从肿瘤所有部位分泌的循环细胞和分子。长链非编码 RNA 分子就是这些分子中的一类。我们进行了一项两阶段的生物标志物研究，研究了新诊断的多发性骨髓瘤（MM）患者、意义未明的单克隆丙种球蛋白血症（MGUS）患者与健康供体（HD）外周血血清中外泌体 lncRNA 表达谱。令人惊讶的是，与 HD 相比，这项分析仅显示 MM 中外泌体 lncRNA PRINS 的表达失调。总体而言，MM 和 MGUS 患者与 HD 患者的区分具有 84.9%的敏感性和 83.3%的特异性。我们的研究提示，外泌体 lncRNA PRINS 可能在单克隆丙种球蛋白血症患者的诊断中发挥作用。
