大肠杆菌的条件致死性脱氧核糖核酸连接酶突变体。
Conditional-lethal deoxyribonucleic acid ligase mutant of Escherichia coli.
作者信息
Dermody J J, Robinson G T, Sternglanz R
出版信息
J Bacteriol. 1979 Aug;139(2):701-4. doi: 10.1128/jb.139.2.701-704.1979.
Abstract
A new Escherichia coli deoxyribonucleic acid (DNA) ligase mutant has been identified among a collection of temperature-sensitive DNA replication mutants isolated recently (Sevastopoulos, Wehr, and Glaser, Proc. Natl. Acad. Sci. U.S.A. 74:3485-3489, 1977). At the nonpermissive temperature DNA synthesis in the mutant stops rapidly, the DNA is degraded to acid-soluble material, and cell death ensures. This suggests that the mutant may be among the most ligase-deficient strains yet characterized.
摘要
在最近分离出的一组温度敏感型DNA复制突变体中，鉴定出了一种新的大肠杆菌脱氧核糖核酸（DNA）连接酶突变体（塞瓦斯托普洛斯、韦尔和格拉泽，《美国国家科学院院刊》74:3485 - 3489，1977）。在非允许温度下，突变体中的DNA合成迅速停止，DNA被降解为酸溶性物质，最终导致细胞死亡。这表明该突变体可能是迄今所鉴定出的连接酶缺陷最严重的菌株之一。
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