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v-myb癌基因产物的核区室化

Nuclear compartmentalization of the v-myb oncogene product.

作者信息

Boyle W J, Lampert M A, Li A C, Baluda M A

出版信息

Mol Cell Biol. 1985 Nov;5(11):3017-23. doi: 10.1128/mcb.5.11.3017-3023.1985.

DOI:10.1128/mcb.5.11.3017-3023.1985
PMID:3018495
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC369114/
Abstract

Nuclei obtained from chicken leukemic myeloblasts transformed by avian myeloblastosis virus were fractionated into various subnuclear compartments, which were then analyzed by specific immunoprecipitation for the presence of the leukemogenic product, p48v-myb, of the viral oncogene. In cells labeled for 30 or 60 min with L-[35S]methionine and in unlabeled exponentially dividing leukemic cells analyzed by Western blotting, p48v-myb was detected within the nucleoplasm (29 +/- 9% [standard deviation] of the total), chromatin (7 +/- 4%), and lamina-nuclear matrix (64 +/- 9%). Also, in myeloblasts analyzed by immunofluorescence during mitosis, p48v-myb appeared to be dispersed through the cell like the lamina-nuclear matrix complex. Strong attachment to the nuclear matrix-lamina complex suggests that p48v-myb may be involved in DNA replication or transcription or both.

摘要

从经禽成髓细胞瘤病毒转化的鸡白血病成髓细胞中获得的细胞核被分离成各种亚核区室，然后通过特异性免疫沉淀分析病毒癌基因的致白血病产物p48v-myb的存在情况。在用L-[35S]甲硫氨酸标记30或60分钟的细胞以及通过蛋白质免疫印迹分析的未标记的指数生长白血病细胞中，在核质（占总量的29±9%[标准差]）、染色质（7±4%）和核纤层-核基质（64±9%）中检测到p48v-myb。此外，在有丝分裂期间通过免疫荧光分析的成髓细胞中，p48v-myb似乎像核纤层-核基质复合物一样分散在整个细胞中。与核基质-核纤层复合物的强烈附着表明p48v-myb可能参与DNA复制或转录或两者皆有。

相似文献

1
Nuclear compartmentalization of the v-myb oncogene product.v-myb癌基因产物的核区室化
Mol Cell Biol. 1985 Nov;5(11):3017-23. doi: 10.1128/mcb.5.11.3017-3023.1985.
2
v-myb does not prevent the expression of c-myb in avian erythroblasts.v-myb并不阻止c-myb在禽类成红细胞中的表达。
J Virol. 1987 Oct;61(10):3284-7. doi: 10.1128/JVI.61.10.3284-3287.1987.
3
Subnuclear localization of proteins encoded by the oncogene v-myb and its cellular homolog c-myb.癌基因v-myb及其细胞同源物c-myb编码蛋白的亚核定位。
Mol Cell Biol. 1986 Jan;6(1):62-9. doi: 10.1128/mcb.6.1.62-69.1986.
4
Structural and functional domains of the myb oncogene: requirements for nuclear transport, myeloid transformation, and colony formation.myb癌基因的结构和功能结构域：核转运、髓系转化和集落形成的要求。
J Virol. 1988 Jun;62(6):1981-8. doi: 10.1128/JVI.62.6.1981-1988.1988.
5
Specific amino acid substitutions are not required for transformation by v-myb of avian myeloblastosis virus.禽成髓细胞瘤病毒的v-myb基因进行转化并不需要特定的氨基酸替换。
J Virol. 1988 Mar;62(3):1093-6. doi: 10.1128/JVI.62.3.1093-1096.1988.
6
Subcellular localization of proteins encoded by oncogenes of avian myeloblastosis virus and avian leukemia virus E26 and by chicken c-myb gene.禽成髓细胞瘤病毒和禽白血病病毒E26的癌基因以及鸡c-myb基因所编码蛋白质的亚细胞定位。
Cell. 1984 Jun;37(2):537-47. doi: 10.1016/0092-8674(84)90384-2.
7
DNA-binding activity associated with the v-myb oncogene product is not sufficient for transformation.与v-myb癌基因产物相关的DNA结合活性不足以引发转化。
J Virol. 1988 Nov;62(11):4398-402. doi: 10.1128/JVI.62.11.4398-4402.1988.
8
env-encoded residues are not required for transformation by p48v-myb.p48v-myb介导的转化不需要env编码的残基。
J Virol. 1987 Mar;61(3):933-6. doi: 10.1128/JVI.61.3.933-936.1987.
9
Mapping of a small phosphopeptide at the carboxyterminus of the viral myb protein by monoclonal antibodies.利用单克隆抗体对病毒myb蛋白羧基末端的一个小磷酸肽进行定位。
Oncogene. 1989 Jan;4(1):33-8.
10
Expression of a truncated v-myb product in transformed chicken embryo fibroblasts.截短的v-myb产物在转化的鸡胚成纤维细胞中的表达。
FEBS Lett. 1987 Apr 6;214(1):81-6. doi: 10.1016/0014-5793(87)80017-0.

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Mol Biol Cell. 1996 Aug;7(8):1299-317. doi: 10.1091/mbc.7.8.1299.
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Human immunodeficiency virus type 1 viral protein R localization in infected cells and virions.1型人类免疫缺陷病毒病毒蛋白R在受感染细胞和病毒颗粒中的定位。
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Regulation of bFGF gene expression and subcellular distribution of bFGF protein in adrenal medullary cells.

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