Howe T R, LaQuier F W, Barbour A G
Infect Immun. 1986 Oct;54(1):207-12. doi: 10.1128/iai.54.1.207-212.1986.
OspA and OspB are major outer membrane proteins and antigens of the Lyme disease spirochete, Borrelia burgdorferi. We examined the organization of ospA and ospB, the genes encoding these proteins. The location and direction of transcription of each osp gene was determined by subcloning, deletion analysis, and transposon Tn5 mutagenesis. Transposon Tn5 insertions within the ospA gene abrogated expression of ospB, suggesting that these genes are transcribed from a common promoter. Northern blot analysis of mRNA from B. burgdorferi with two DNA probes individually specific for ospA or ospB identified a 2.2-kilobase transcript that hybridized with each probe. These studies indicate that the two osp genes of B. burgdorferi constitute a single transcriptional unit.
OspA和OspB是莱姆病螺旋体伯氏疏螺旋体的主要外膜蛋白和抗原。我们研究了ospA和ospB（编码这些蛋白质的基因）的组织结构。通过亚克隆、缺失分析和转座子Tn5诱变确定了每个osp基因的转录位置和方向。ospA基因内的转座子Tn5插入消除了ospB的表达，这表明这些基因是从一个共同的启动子转录而来的。用分别对ospA或ospB特异的两种DNA探针，对来自伯氏疏螺旋体的mRNA进行Northern印迹分析，鉴定出一个与每种探针杂交的2.2千碱基转录本。这些研究表明，伯氏疏螺旋体的两个osp基因构成一个单一的转录单元。
