Department of Chemistry and Bioscience, Aalborg University, 9100 Aalborg, Denmark.
Department of Chemistry and Bioscience, Aalborg University, 6700 Esbjerg, Denmark.
Molecules. 2018 Sep 2;23(9):2232. doi: 10.3390/molecules23092232.
Genome sequencing of the genus has revealed a great capacity for discovery of new natural products of potential economical and therapeutic importance. Several of these are unknown. In this study, we investigated the product of the gene in , which was recently linked to gibepyrones in . Genomic analyses showed that constitutes a stand-alone gene in and related species. Overexpression of resulted in production of gibepyrones A, B, D, G and prolipyrone B, which could not be detected in the wild type strain. Our results suggest that produces the entry compound gibepyrone A, which is subsequently oxidized by one or several non-clustering cytochrome P450 monooxygenases ending with prolipyrone B.
对 属的基因组测序揭示了其具有发现具有潜在经济和治疗重要性的新型天然产物的巨大潜力。其中有几种是未知的。在这项研究中，我们研究了最近与 中的 gibepyrones 相关的 基因的产物。基因组分析表明， 在 和相关物种中构成独立的基因。 基因的过表达导致 gibepyrones A、B、D、G 和 prolipyrone B 的产生，而在野生型菌株中无法检测到这些产物。我们的结果表明， 产生 entry compound gibepyrone A，随后被一个或多个非聚类细胞色素 P450 单加氧酶氧化，最终生成 prolipyrone B。
