Ennis F A, Martin W J, Verbonitz M W
J Exp Med. 1977 Sep 1;146(3):893-8. doi: 10.1084/jem.146.3.893.
Specific cytotoxic thymus-derived (T) lymphocytes were detected in the cervical lymph nodes and spleen during influenza infection of mice. The cytotoxic T cells can distinguish target cells infected with different influenza A subtypes. Infection with parent viruses and their recombinant progeny possessing the hemagglutinin of one parent and the neuraminidase of the other demonstrated that significant cytotoxicity occurred only when the hemagglutinin of the immunizing viruses was the same as that of the virus used to infect the target cell. In addition to this specific cytotoxic response to the major surface antigen, a cross-reactive response could be detected when the relatively nonpermissive L cell was used as the target cell. These results indicate there is a specific cytotoxic T-cell response to the surface hemagglutinin, and a cross-reactive cytotoxic response, not directed to the hemagglutinin, during influenza infection. The cytotoxic T-cell response specific for the hemagglutinin antigen may play an important role in in vivo immunity to influenza.
在小鼠流感感染期间，在颈部淋巴结和脾脏中检测到了特异性细胞毒性胸腺来源的（T）淋巴细胞。细胞毒性T细胞能够区分感染不同甲型流感病毒亚型的靶细胞。用具有一种亲本血凝素和另一种亲本神经氨酸酶的亲本病毒及其重组子代病毒进行感染实验，结果表明，只有当免疫病毒的血凝素与用于感染靶细胞的病毒的血凝素相同时，才会出现显著的细胞毒性。除了对主要表面抗原的这种特异性细胞毒性反应外，当使用相对不易感染的L细胞作为靶细胞时，还能检测到交叉反应性反应。这些结果表明，在流感感染期间，存在针对表面血凝素的特异性细胞毒性T细胞反应，以及一种并非针对血凝素的交叉反应性细胞毒性反应。针对血凝素抗原的细胞毒性T细胞反应可能在体内抗流感免疫中发挥重要作用。
