Liu Yi, Ghassemi Pejhman, Depkon Andrew, Iacono Maria Ida, Lin Jonathan, Mendoza Gonzalo, Wang Jianting, Tang Qinggong, Chen Yu, Pfefer T Joshua
Department of Bioengineering, University of Maryland, Silver Spring, MD, USA.
Center for Devices and Radiological Health, U.S. Food and Drug Administration, Silver Spring, MD, USA.
Biomed Opt Express. 2018 May 29;9(6):2810-2824. doi: 10.1364/BOE.9.002810. eCollection 2018 Jun 1.
Emerging three-dimensional (3D) printing technology enables the fabrication of optically realistic and morphologically complex tissue-simulating phantoms for the development and evaluation of novel optical imaging products. In this study, we assess the potential to print image-defined neurovascular phantoms with patent channels for contrast-enhanced near-infrared fluorescence (NIRF) imaging. An anatomical map defined from clinical magnetic resonance imaging (MRI) was segmented and processed into files suitable for printing a forebrain vessel network in rectangular and curved-surface biomimetic phantoms. Methods for effectively cleaning samples with complex vasculature were determined. A final set of phantoms were imaged with a custom NIRF system at 785 nm excitation using two NIRF contrast agents. In addition to demonstrating the strong potential of 3D printing for creating highly realistic, patient-specific biophotonic phantoms, our work provides insight into optimal methods for accomplishing this goal and elucidates current limitations of this approach.
新兴的三维（3D）打印技术能够制造出光学逼真且形态复杂的组织模拟体模，用于新型光学成像产品的开发和评估。在本研究中，我们评估了打印具有专利通道的图像定义神经血管体模以进行对比增强近红外荧光（NIRF）成像的潜力。从临床磁共振成像（MRI）定义的解剖图谱被分割并处理成适合在矩形和曲面仿生体模中打印前脑血管网络的文件。确定了有效清洁具有复杂脉管系统样本的方法。最后一组体模使用两种NIRF造影剂在785 nm激发下用定制的NIRF系统进行成像。除了展示3D打印在创建高度逼真、针对患者的生物光子体模方面的巨大潜力外，我们的工作还深入探讨了实现这一目标的最佳方法，并阐明了这种方法目前的局限性。
