Jo Ara, Kim Jeongjin, Ding Tian, Ahn Juhee
1Department of Medical Biomaterials Engineering and Institute of Bioscience and Biotechnology, Kangwon National University, Chuncheon, Gangwon, 24341 Korea.
2Department of Food Science and Nutrition, Zhejiang Key Laboratory for Agro-Food Processing, Zhejiang University, Hangzhou, Zhejiang, 310058 China.
Food Sci Biotechnol. 2016 Aug 31;25(4):1211-1215. doi: 10.1007/s10068-016-0192-6. eCollection 2016.
This study was designed to evaluate the effect of phage-antibiotic synergy in reducing antibiotic resistance. The initial numbers of treated with ciprofloxacin, phages, and combination were significantly reduced by 3.47, 4.62, and 5.75 log CFU/mL, respectively, at the early 12 h of incubation. The combination treatment most effectively inhibited the growth of , showing more than 4 log reduction in 18 h of incubation at 37°C. The significant reduction in biofilm formation by was observed at the combination treatment (3.91 log). Ciprofloxacin-treated cells became resistant to both ciprofloxacin and phage, showing the mutant frequencies of 27% and 25%, respectively, whereas no antibiotic- and phage-resistant cells were observed at the combined treatment of ciprofloxacin and phages. These results provide useful information for reducing the risk of antibiotic resistance in human and food animals.
本研究旨在评估噬菌体 - 抗生素协同作用在降低抗生素耐药性方面的效果。在培养的最初12小时，用环丙沙星、噬菌体及联合处理的初始菌数分别显著减少了3.47、4.62和5.75 log CFU/mL。联合处理最有效地抑制了[细菌名称未给出]的生长，在37°C培养18小时时显示出超过4 log的减少。联合处理时观察到[细菌名称未给出]生物膜形成显著减少（3.91 log）。用环丙沙星处理的[细菌名称未给出]细胞对环丙沙星和噬菌体均产生耐药性，突变频率分别为27%和25%，而在环丙沙星和噬菌体联合处理时未观察到抗抗生素和抗噬菌体的[细菌名称未给出]细胞。这些结果为降低人和食用动物抗生素耐药性风险提供了有用信息。
