Liang Jin, Yan Hua, Yang Han-Joo, Kim Hye Won, Wan Xiaochun, Lee Jinhee, Ko Sanghoon
1State Key Laboratory of Tea Plant Biology and Utilization, Anhui Agricultural University, Hefei, 230036 China.
2Department of Food Science and Biotechnology, Sejong University, Seoul, 05006 Korea.
Food Sci Biotechnol. 2016 Dec 31;25(6):1583-1590. doi: 10.1007/s10068-016-0244-y. eCollection 2016.
A nano-sized double-walled carrier composed of chitosan and β-lactoglobulin (β-Lg) for oral administration of epigallocatechin gallate (EGCG) was developed to achieve a prolonged release of EGCG in the gastrointestinal tract. Carboxymethyl chitosan (CMC) solution was added dropwise to chitosan hydrochloride (CHC) containing EGCG to form a primary coating by ionic complexation. Subsequently, β-Lg was added to create a secondary layer by ionic gelation. The obtained EGCG-loaded chitosan/β-Lg nanoparticles had sizes between 100 and 500 nm and zeta potentials ranging from 10 to 35mV. FT-IR spectroscopy revealed a high number of hydrogen-bonding sites in the nanoparticles, which could incorporate EGCG, resulting in high encapsulation efficiency. EGCG incorporated in the primary coating was released slowly over time by diffusion from the swollen CMC-CHC matrix after the outer layer of β-Lg was degraded in the intestinal fluid. The sustained-release property makes chitosan/β-Lg nanoparticles an attractive candidate for effective delivery of EGCG.
开发了一种由壳聚糖和β-乳球蛋白（β-Lg）组成的纳米级双壁载体，用于口服表没食子儿茶素没食子酸酯（EGCG），以实现EGCG在胃肠道中的缓释。将羧甲基壳聚糖（CMC）溶液滴加到含有EGCG的壳聚糖盐酸盐（CHC）中，通过离子络合形成初级包衣。随后，加入β-Lg通过离子凝胶化形成第二层。所制备的负载EGCG的壳聚糖/β-Lg纳米颗粒尺寸在100至500nm之间，ζ电位在10至35mV范围内。傅里叶变换红外光谱（FT-IR）显示纳米颗粒中有大量氢键位点，可结合EGCG，从而实现高包封率。在肠道液中β-Lg外层降解后，初级包衣中包载的EGCG通过从溶胀的CMC-CHC基质中扩散而随时间缓慢释放。这种缓释特性使壳聚糖/β-Lg纳米颗粒成为有效递送EGCG的有吸引力的候选物。
