从 RNA 阳性但血清学阴性的血浆供体中分离出的戊型肝炎病毒 3 型基因组，其 ORF3 的起始密码子为 CUG。
Hepatitis E Virus Genotype 3 Genomes from RNA-Positive but Serologically Negative Plasma Donors Have CUG as the Start Codon for ORF3.
机构信息
Department of Infectious Diseases, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Department of Biomedical Sciences for Health, University of Milan, Milan, Italy.
出版信息
Intervirology. 2018;61(2):96-103. doi: 10.1159/000491926. Epub 2018 Oct 2.
Abstract
Hepatitis E virus (HEV) is a pathogen that causes hepatitis worldwide. Molecular studies have identified HEV RNA in blood products although its significance is not understood. This study was undertaken to characterize HEV genomes in asymptomatic plasma donors from Sweden and Germany lacking anti-HEV. Complete open reading frames (ORFs) were obtained from HEV strains in 5 out of 18 plasma donors who tested positive for HEV RNA. All strains had CUG as the start codon of ORF3, while 147 GenBank strains all had AUG as the start codon (p < 0.0001). This substitution was found in both interrelated and unrelated strains belonging to different phylogenetic clades. The HEV strains from the seronegative plasma donors had no other substitution in common, which may be why the CUG substitution seems to explain the seronegativity.
摘要
戊型肝炎病毒 (HEV) 是一种引起全球肝炎的病原体。分子研究已经在血液制品中发现了 HEV RNA，但其意义尚不清楚。本研究旨在描述来自瑞典和德国的无症状血浆供体中的 HEV 基因组，这些供体缺乏抗-HEV。从 18 名 RNA 检测呈阳性的血浆供体中，有 5 名获得了 HEV 病毒株的完整开放阅读框 (ORF)。所有毒株的 ORF3 起始密码子均为 CUG，而 147 株 GenBank 毒株的起始密码子均为 AUG (p < 0.0001)。这种替代发生在属于不同系统发育分支的相互关联和不相关的毒株中。来自血清阴性血浆供体的 HEV 株没有其他共同的替代，这可能就是为什么 CUG 替代似乎可以解释血清阴性。
相似文献
1
Hepatitis E Virus Genotype 3 Genomes from RNA-Positive but Serologically Negative Plasma Donors Have CUG as the Start Codon for ORF3.从 RNA 阳性但血清学阴性的血浆供体中分离出的戊型肝炎病毒 3 型基因组，其 ORF3 的起始密码子为 CUG。
Intervirology. 2018;61(2):96-103. doi: 10.1159/000491926. Epub 2018 Oct 2.
2
Genetic characterization and codon usage bias of full-length Hepatitis E virus sequences shed new lights on genotypic distribution, host restriction and genome evolution.全长丙型肝炎病毒序列的遗传特征和密码子使用偏性为基因型分布、宿主限制和基因组进化提供了新的线索。
Infect Genet Evol. 2012 Dec;12(8):1842-53. doi: 10.1016/j.meegid.2012.07.021. Epub 2012 Aug 21.
3
Prevalence of antibodies to hepatitis E virus among Japanese blood donors: identification of three blood donors infected with a genotype 3 hepatitis E virus.日本献血者中戊型肝炎病毒抗体的流行情况：三名感染3型戊型肝炎病毒的献血者的鉴定
J Med Virol. 2004 Aug;73(4):554-61. doi: 10.1002/jmv.20125.
4
Analysis of the complete genome sequences of one swine and two human hepatitis E virus genotype 4 strains isolated in Beijing, China.分析在中国北京分离到的一株猪源和两株人源戊型肝炎病毒 4 型的全基因组序列。
Infect Genet Evol. 2013 Aug;18:42-7. doi: 10.1016/j.meegid.2013.04.038. Epub 2013 May 16.
5
Identification of two distinct genotypes of hepatitis E virus in a Japanese patient with acute hepatitis who had not travelled abroad.在一名未曾出过国的日本急性肝炎患者中鉴定出两种不同基因型的戊型肝炎病毒。
J Gen Virol. 2002 Aug;83(Pt 8):1931-1940. doi: 10.1099/0022-1317-83-8-1931.
6
High prevalence of anti-hepatitis E virus antibodies among blood donors in central Italy, February to March 2014.2014 年 2 月至 3 月，意大利中部献血者中抗戊型肝炎病毒抗体的高流行率。
Euro Surveill. 2016 Jul 28;21(30). doi: 10.2807/1560-7917.ES.2016.21.30.30299.
7
Ongoing subclinical infection of hepatitis E virus among blood donors with an elevated alanine aminotransferase level in Japan.日本丙氨酸转氨酶水平升高的献血者中戊型肝炎病毒的持续亚临床感染
J Med Virol. 2007 Jun;79(6):734-42. doi: 10.1002/jmv.20834.
8
Analysis of complete genome sequences and a V239A substitution in the helicase domain of swine hepatitis E virus strains isolated in Canada.对在加拿大分离的猪戊型肝炎病毒株的全基因组序列及解旋酶结构域中的V239A替换进行分析。
Arch Virol. 2015 Jul;160(7):1767-73. doi: 10.1007/s00705-015-2429-8. Epub 2015 Apr 29.
9
Primary structure of open reading frame 2 and 3 of the hepatitis E virus isolated from Morocco.从摩洛哥分离出的戊型肝炎病毒开放阅读框2和3的一级结构。
J Med Virol. 1999 Feb;57(2):126-33.
10
[Hepatitis E virus infection in population of non-remunerate blood donors in Shaoxing].[绍兴市无偿献血人群戊型肝炎病毒感染情况]
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2007 Mar;21(1):29-31.
引用本文的文献
1
Molecular Identification and Characterization of a Genotype 3 Hepatitis E Virus (HEV) Strain Detected in a Wolf Faecal Sample, Italy.在意大利一份狼粪便样本中检测到的3型戊型肝炎病毒（HEV）毒株的分子鉴定与特征分析
Animals (Basel). 2021 Dec 5;11(12):3465. doi: 10.3390/ani11123465.
2
Evaluation of hepatitis E antigen kinetics and its diagnostic utility for prediction of the outcomes of hepatitis E virus genotype 1 infection.评价戊型肝炎病毒 1 型感染患者的肝炎 E 抗原动力学及其对预测结局的诊断价值。
Virulence. 2021 Dec;12(1):1334-1344. doi: 10.1080/21505594.2021.1922027.
3
Classification of the Zoonotic Hepatitis E Virus Genotype 3 Into Distinct Subgenotypes.人畜共患戊型肝炎病毒3型向不同亚基因型的分类。
Front Microbiol. 2021 Jan 28;11:634430. doi: 10.3389/fmicb.2020.634430. eCollection 2020.
4
Role of Hepatitis E Virus Infection in North American Patients With Severe Acute Liver Injury.戊型肝炎病毒感染在北美的严重急性肝损伤患者中的作用。
Clin Transl Gastroenterol. 2020 Nov;11(11):e00273. doi: 10.14309/ctg.0000000000000273.
5
Variations among Viruses in Influent Water and Effluent Water at a Wastewater Plant over One Year as Assessed by Quantitative PCR and Metagenomics.定量 PCR 和宏基因组学评估一年中污水处理厂进水中和出水中病毒的变化。
Appl Environ Microbiol. 2020 Nov 24;86(24). doi: 10.1128/AEM.02073-20.
6
Hepatitis E virus is an infrequent but potentially serious infection in allogeneic hematopoietic stem cell transplant recipients.戊型肝炎病毒在异基因造血干细胞移植受者中是一种不常见但可能严重的感染。
Bone Marrow Transplant. 2020 Jul;55(7):1255-1263. doi: 10.1038/s41409-020-0823-7. Epub 2020 Feb 18.
7
High Frequency of Either Altered Pre-Core StartCodon or Weakened Kozak Sequence in the CorePromoter Region in Hepatitis B Virus A1 Strainsfrom Rwanda.卢旺达乙型肝炎病毒 A1 株中核心启动子区前核心起始密码子改变或 Kozak 序列减弱的高频发生。
Genes (Basel). 2019 Feb 26;10(3):182. doi: 10.3390/genes10030182.
本文引用的文献
1
Differential removal of human pathogenic viruses from sewage by conventional and ozone treatments.常规处理和臭氧处理对污水中人类致病病毒的去除效果差异。
Int J Hyg Environ Health. 2018 Apr;221(3):479-488. doi: 10.1016/j.ijheh.2018.01.012. Epub 2018 Feb 1.
2
Seroprevalence of hepatitis E virus among blood donors in northern Italy (Sondrio, Lombardy) determined by three different assays.采用三种不同检测方法测定意大利北部（松德里奥省、伦巴第大区）献血者中戊型肝炎病毒的血清流行率。
Blood Transfus. 2017 Oct;15(6):502-505. doi: 10.2450/2017.0089-17.
3
ICTV Virus Taxonomy Profile: Hepeviridae.国际病毒分类委员会病毒分类概况：戊型肝炎病毒科
J Gen Virol. 2017 Nov;98(11):2645-2646. doi: 10.1099/jgv.0.000940. Epub 2017 Oct 12.
4
Hepatitis E viral infection in solid organ transplant patients.实体器官移植患者中的戊型肝炎病毒感染
Curr Opin Organ Transplant. 2017 Aug;22(4):351-355. doi: 10.1097/MOT.0000000000000432.
5
Hepatitis E and blood donation safety in selected European countries: a shift to screening?部分欧洲国家的戊型肝炎与献血安全：是否转向筛查？
Euro Surveill. 2017 Apr 20;22(16). doi: 10.2807/1560-7917.ES.2017.22.16.30514.
6
Cost-effectiveness of the screening of blood donations for hepatitis E virus in the Netherlands.荷兰戊型肝炎病毒献血筛查的成本效益
Transfusion. 2017 Feb;57(2):258-266. doi: 10.1111/trf.13978. Epub 2017 Jan 31.
7
Hepatitis E virus ORF3 is a functional ion channel required for release of infectious particles.戊型肝炎病毒开放阅读框3是释放感染性颗粒所必需的功能性离子通道。
Proc Natl Acad Sci U S A. 2017 Jan 31;114(5):1147-1152. doi: 10.1073/pnas.1614955114. Epub 2017 Jan 17.
8
Markers for Ongoing or Previous Hepatitis E Virus Infection Are as Common in Wild Ungulates as in Humans in Sweden.在瑞典，正在感染或既往感染戊型肝炎病毒的标志物在野生有蹄类动物中与在人类中一样常见。
Viruses. 2016 Sep 19;8(9):259. doi: 10.3390/v8090259.
9
Hepatitis E in blood donors: investigation of the natural course of asymptomatic infection, Germany, 2011.2011年德国献血者中的戊型肝炎：无症状感染自然病程调查
Euro Surveill. 2016 Sep 1;21(35). doi: 10.2807/1560-7917.ES.2016.21.35.30332.
10
Low transfusion transmission of hepatitis E among 25,637 single-donation, nucleic acid-tested blood donors.在25637名单次献血且经过核酸检测的献血者中，戊型肝炎的输血传播率较低。
Transfusion. 2016 Sep;56(9):2225-32. doi: 10.1111/trf.13700. Epub 2016 Jul 7.
Zotero 插件