State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
Key Laboratory of Clinical In Vitro Diagnostic Techniques of Zhejiang Province, Department of Clinical Laboratory, the First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, Zhejiang, China.
PLoS One. 2018 Oct 4;13(10):e0204892. doi: 10.1371/journal.pone.0204892. eCollection 2018.
Vibrio parahaemolyticus is a leading foodborne pathogen in southeastern China. In this study, 105 strains of V. parahaemolyticus were isolated from fresh seafood in 2013 and 2014. The serotypes, virulence-associated genes and sequence types (STs) of these strains were analyzed. 26 defined serotypes were identified and 69 strains (65.7%) had untypeable O or K antigen. 8 strains (7.6%) had the virulence-associated gene tdh and no strain carried the trh gene. 45.7% (48/105) of isolates contained all four T3SS1 genes and 50% (4/8) tdh+ trh- V. parahaemolyticus isolates lacked at least one of the four tested T3SS2α genes. 105 strains could be categorized into 84 STs and only 3 STs (ST3, ST8, ST675) had appeared in clinical strains. V. parahaemolyticus strains from seafood have more diverse and untypeable serotypes, less virulence-associated genes and more STs than strains from clinical sources.
副溶血性弧菌是中国东南部地区主要的食源性致病菌。本研究于 2013 年和 2014 年从新鲜海产品中分离出 105 株副溶血性弧菌，分析了这些菌株的血清型、毒力相关基因和序列型（ST）。鉴定出 26 个明确的血清型，69 株（65.7%）的 O 或 K 抗原不可分型。8 株（7.6%）携带毒力相关基因 tdh，没有菌株携带 trh 基因。45.7%（48/105）的分离株含有所有 4 个 T3SS1 基因，50%（4/8）的 tdh+ trh-副溶血性弧菌分离株至少缺少 4 个测试的 T3SS2α基因中的一个。105 株可分为 84 个 ST，只有 3 个 ST（ST3、ST8、ST675）出现在临床菌株中。与临床来源的菌株相比，来自海产品的副溶血性弧菌菌株具有更多样化和不可分型的血清型、较少的毒力相关基因和更多的 ST。
