弯曲DNA片段凝胶迁移异常的温度和盐依赖性
Temperature and salt dependence of the gel migration anomaly of curved DNA fragments.
作者信息
Diekmann S
出版信息
Nucleic Acids Res. 1987 Jan 12;15(1):247-65. doi: 10.1093/nar/15.1.247.
Abstract
A series of oligonucleotides of different sequences have been cloned to study DNA curvature. Several DNA fragments containing these oligonucleotides in various numbers of repeats were analyzed in 10% polyacrylamide gels. A strong gel migration anomaly was found for dA4 sequences; a comparably very small but clearly detectable anomaly was observed for dA3 (both in a repeat length of 10 base-pairs). The temperature and salt (NaCl, MgCl2) dependence of the gel migration anomaly of these DNA fragments was measured. While a similar behaviour of all sequences is observed for the addition of NaCl, the temperature and MgCl2 dependence of the anomaly varies with the oligonucleotide sequence. These data are interpreted in terms of local DNA structure changes induced by changes in the temperature and the MgCl2 concentration which affect the planarity of the curved DNA fragments.
摘要
已克隆了一系列不同序列的寡核苷酸以研究DNA弯曲。在10%的聚丙烯酰胺凝胶中分析了几个含有不同重复次数这些寡核苷酸的DNA片段。发现dA4序列存在强烈的凝胶迁移异常；对于dA3（重复长度均为10个碱基对）观察到相对非常小但明显可检测到的异常。测量了这些DNA片段凝胶迁移异常的温度和盐（NaCl、MgCl2）依赖性。虽然添加NaCl时所有序列表现出相似行为，但异常的温度和MgCl2依赖性随寡核苷酸序列而变化。这些数据根据温度和MgCl2浓度变化引起的局部DNA结构变化来解释，这些变化会影响弯曲DNA片段的平面性。
相似文献
1
Temperature and salt dependence of the gel migration anomaly of curved DNA fragments.弯曲DNA片段凝胶迁移异常的温度和盐依赖性
Nucleic Acids Res. 1987 Jan 12;15(1):247-65. doi: 10.1093/nar/15.1.247.
2
The migration anomaly of DNA fragments in polyacrylamide gels allows the detection of small sequence-specific DNA structure variations.聚丙烯酰胺凝胶中DNA片段的迁移异常能够检测到小的序列特异性DNA结构变异。
Electrophoresis. 1989 May-Jun;10(5-6):354-9. doi: 10.1002/elps.1150100513.
3
The anomalous gel migration of a stable cruciform: temperature and salt dependence, and some comparisons with curved DNA.
Nucleic Acids Res. 1987 Jul 24;15(14):5765-74. doi: 10.1093/nar/15.14.5765.
4
[The effect of temperature and ionic strength on the electrophoretic motility of synthetic DNA fragments].[温度和离子强度对合成DNA片段电泳迁移率的影响]
Mol Biol (Mosk). 1990 Jan-Feb;24(1):79-95.
5
Physiological concentration of magnesium ions induces a strong macroscopic curvature in GGGCCC-containing DNA.镁离子的生理浓度会在含有GGGCCC的DNA中诱导出强烈的宏观曲率。
J Mol Biol. 1994 Feb 11;236(1):26-32. doi: 10.1006/jmbi.1994.1115.
6
Effects of 5 cytosine methylation on the B-Z transition in DNA restriction fragments and recombinant plasmids.5-胞嘧啶甲基化对DNA限制性片段和重组质粒中B-Z转变的影响。
J Mol Biol. 1983 Jul 25;168(1):51-71. doi: 10.1016/s0022-2836(83)80322-2.
7
Sequence specificity of curved DNA.弯曲DNA的序列特异性。
FEBS Lett. 1986 Jan 20;195(1-2):53-6. doi: 10.1016/0014-5793(86)80128-4.
8
DNA fragments with specific nucleotide sequences in their single-stranded termini exhibit unusual electrophoretic mobilities.在其单链末端具有特定核苷酸序列的DNA片段表现出异常的电泳迁移率。
Nucleic Acids Res. 1998 Apr 15;26(8):1899-905. doi: 10.1093/nar/26.8.1899.
9
On the sequence determinants and flexibility of the kinetoplast DNA fragment with abnormal gel electrophoretic mobilities.关于具有异常凝胶电泳迁移率的动质体DNA片段的序列决定因素和灵活性
J Mol Biol. 1985 Nov 5;186(1):1-11. doi: 10.1016/0022-2836(85)90251-7.
10
Suppression of electrophoretic anomaly of bent DNA segments by the structural property that causes rapid migration.通过导致快速迁移的结构特性抑制弯曲DNA片段的电泳异常。
Nucleic Acids Res. 1998 Nov 1;26(21):4811-7. doi: 10.1093/nar/26.21.4811.
引用本文的文献
1
Examining the Effects of Netropsin on the Curvature of DNA A-Tracts Using Electrophoresis.使用电泳技术研究 Netropsin 对 DNA A- 链段曲率的影响。
Molecules. 2021 Sep 28;26(19):5871. doi: 10.3390/molecules26195871.
2
New Aspects of Magnesium Function: A Key Regulator in Nucleosome Self-Assembly, Chromatin Folding and Phase Separation.镁功能的新方面：核小体自组装、染色质折叠和相分离的关键调节剂。
Int J Mol Sci. 2019 Aug 29;20(17):4232. doi: 10.3390/ijms20174232.
3
Divergence of VRN-B3 alleles during the evolution of domesticated wheat.驯化小麦进化过程中 VRN-B3 等位基因的分歧。
Mol Genet Genomics. 2019 Feb;294(1):263-275. doi: 10.1007/s00438-018-1506-6. Epub 2018 Oct 29.
4
DNA recognition by a σ(54) transcriptional activator from Aquifex aeolicus.嗜热栖热菌中一种σ(54)转录激活因子对DNA的识别
J Mol Biol. 2014 Oct 23;426(21):3553-68. doi: 10.1016/j.jmb.2014.08.009. Epub 2014 Aug 23.
5
Structural determinants of DNA recognition by plant MADS-domain transcription factors.植物 MADS 结构域转录因子识别 DNA 的结构决定因素。
Nucleic Acids Res. 2014 Feb;42(4):2138-46. doi: 10.1093/nar/gkt1172. Epub 2013 Nov 25.
6
The pneumococcal MgaSpn virulence transcriptional regulator generates multimeric complexes on linear double-stranded DNA.肺炎链球菌 MgaSpn 毒力转录调节因子在线性双链 DNA 上形成多聚体复合物。
Nucleic Acids Res. 2013 Aug;41(14):6975-91. doi: 10.1093/nar/gkt445. Epub 2013 May 30.
7
DNA A-tracts are not curved in solutions containing high concentrations of monovalent cations.在含有高浓度单价阳离子的溶液中，DNA A-链段没有弯曲。
Biochemistry. 2013 Jun 18;52(24):4138-48. doi: 10.1021/bi400118m. Epub 2013 Jun 6.
8
Segrosome assembly at the pliable parH centromere.柔性 ParH 着丝粒处的 Segrosome 组装。
Nucleic Acids Res. 2011 Jul;39(12):5082-97. doi: 10.1093/nar/gkr115. Epub 2011 Mar 4.
9
The DNA sequence-dependence of nucleosome positioning in vivo and in vitro.体内和体外核小体定位的 DNA 序列依赖性。
J Biomol Struct Dyn. 2010 Jun;27(6):713-24. doi: 10.1080/073911010010524942.
10
A measure of bending in nucleic acids structures applied to A-tract DNA.一种应用于 A 型 DNA 的核酸结构弯曲程度的测量方法。
Nucleic Acids Res. 2010 Jun;38(10):3414-22. doi: 10.1093/nar/gkq001. Epub 2010 Jan 31.
本文引用的文献
1
Bent helical structure in kinetoplast DNA.动基体 DNA 的弯曲螺旋结构。
Proc Natl Acad Sci U S A. 1982 Dec;79(24):7664-8. doi: 10.1073/pnas.79.24.7664.
2
The pitch of chromatin DNA is reflected in its nucleotide sequence.染色质DNA的螺距反映在其核苷酸序列中。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3816-20. doi: 10.1073/pnas.77.7.3816.
3
Filter disc supported oligonucleotide synthesis by the phosphite method.滤膜支持的亚磷酸酯法寡核苷酸合成。
Nucleic Acids Res. 1984 Dec 11;12(23):9137-42. doi: 10.1093/nar/12.23.9137.
4
The locus of sequence-directed and protein-induced DNA bending.序列导向和蛋白质诱导的DNA弯曲位点。
Nature. 1984;308(5959):509-13. doi: 10.1038/308509a0.
5
Anomalous electrophoresis of deoxyribonucleic acid restriction fragments on polyacrylamide gels.脱氧核糖核酸限制片段在聚丙烯酰胺凝胶上的异常电泳。
Biochemistry. 1983 Dec 20;22(26):6186-93. doi: 10.1021/bi00295a023.
6
Sequencing end-labeled DNA with base-specific chemical cleavages.通过碱基特异性化学切割对末端标记的DNA进行测序。
Methods Enzymol. 1980;65(1):499-560. doi: 10.1016/s0076-6879(80)65059-9.
7
Evidence for the existence of stable curvature of DNA in solution.溶液中DNA存在稳定曲率的证据。
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4632-6. doi: 10.1073/pnas.81.15.4632.
8
Sequence dependence of the curvature of DNA: a test of the phasing hypothesis.DNA曲率的序列依赖性：相位假说的检验
Biochemistry. 1985 Dec 3;24(25):7033-7. doi: 10.1021/bi00346a001.
9
DNA bending at adenine . thymine tracts.腺嘌呤·胸腺嘧啶序列处的DNA弯曲
Nature. 1986;320(6062):501-6. doi: 10.1038/320501a0.
10
DNA structure. Bent molecules--how and why?
Nature. 1986;320(6062):487-8. doi: 10.1038/320487a0.
Zotero 插件