School of Food Science and Engineering, South China University of Technology, Guangzhou 510640, China.
Guangdong Province Key Laboratory for Green Processing of Natural Products and Product Safety, Guangzhou 510640, China.
Molecules. 2018 Oct 10;23(10):2592. doi: 10.3390/molecules23102592.
Bladder cancer has become the most common malignant urinary carcinoma. Studies have shown that significant antioxidant and bladder cancer-fighting properties of several plant-based diets like , ginger and amomum, are associated with their high kaempferol content. In this paper, we evaluated the antioxidant and anticancer activities of kaempferol and its mechanism of induction to apoptosis on bladder cancer cells. Our findings demonstrated that kaempferol showed an obvious radical scavenging activity in erythrocytes damaged by oxygen. Kaempferol promoted antioxidant enzymes, inhibited ROS generation and lipid peroxidation and finally prevented the occurrence of hemolysis. Additionally, kaempferol exhibited a strong inhibitory effect on bladder cancer cells and high safety on normal bladder cells. At the molecular level, kaempferol suppressed EJ bladder cancer cell proliferation by inhibiting the function of phosphorylated AKT (p-AKT), CyclinD1, CDK4, Bid, Mcl-1 and Bcl-xL, and promoting p-BRCA1, p-ATM, p53, p21, p38, Bax and Bid expression, and finally triggering apoptosis and S phase arrest. We found that Kaempferol exhibited strong anti-oxidant activity on erythrocyte and inhibitory effects on the growth of cancerous bladder cells through inducing apoptosis and S phase arrest. These findings suggested that kaempferol might be regarded as a bioactive food ingredient to prevent oxidative damage and treat bladder cancer.
膀胱癌已成为最常见的恶性尿路上皮癌。研究表明，一些植物性饮食如生姜和砂仁具有显著的抗氧化和抗癌特性，这与其高含量的山柰酚有关。在本文中，我们评估了山柰酚的抗氧化和抗癌活性及其诱导膀胱癌细胞凋亡的机制。我们的研究结果表明，山柰酚在氧损伤的红细胞中表现出明显的自由基清除活性。山柰酚能促进抗氧化酶的活性，抑制 ROS 的产生和脂质过氧化，最终防止溶血的发生。此外，山柰酚对膀胱癌细胞具有很强的抑制作用，对正常膀胱细胞具有很高的安全性。在分子水平上，山柰酚通过抑制磷酸化 AKT（p-AKT）、CyclinD1、CDK4、Bid、Mcl-1 和 Bcl-xL 的功能，促进 p-BRCA1、p-ATM、p53、p21、p38、Bax 和 Bid 的表达，从而抑制 EJ 膀胱癌细胞的增殖，最终引发细胞凋亡和 S 期阻滞。我们发现山柰酚通过诱导细胞凋亡和 S 期阻滞，对红细胞具有很强的抗氧化活性，对癌细胞生长有抑制作用。这些发现表明，山柰酚可能被视为一种具有生物活性的食物成分，可预防氧化损伤和治疗膀胱癌。
