CRISPR-PLANT v2：一个基于改进的脱靶分析提供高度特异性向导RNA间隔序列的在线资源。 - Suppr

CRISPR-PLANT v2: an online resource for highly specific guide RNA spacers based on improved off-target analysis.

作者信息

Minkenberg Bastian, Zhang Jianwei, Xie Kabin, Yang Yinong

机构信息

Intercollege Graduate Degree Program in Plant Biology, The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, State College, PA, USA.

Department of Plant Pathology and Environmental Microbiology, The Pennsylvania State University, University Park, State College, PA, USA.

出版信息

Plant Biotechnol J. 2019 Jan;17(1):5-8. doi: 10.1111/pbi.13025. Epub 2018 Dec 13.

DOI:10.1111/pbi.13025

PMID:30325102

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6330547/

Abstract

摘要

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5040/11386470/f4716b9f5724/PBI-17-5-g001.jpg

相似文献

CRISPR-PLANT v2: an online resource for highly specific guide RNA spacers based on improved off-target analysis.

Plant Biotechnol J. 2019 Jan;17(1):5-8. doi: 10.1111/pbi.13025. Epub 2018 Dec 13.

CRISPR GUARD protects off-target sites from Cas9 nuclease activity using short guide RNAs.

Nat Commun. 2020 Aug 17;11(1):4132. doi: 10.1038/s41467-020-17952-5.

Advances in detecting and reducing off-target effects generated by CRISPR-mediated genome editing.

J Genet Genomics. 2019 Nov 20;46(11):513-521. doi: 10.1016/j.jgg.2019.11.002. Epub 2019 Nov 22.

SeqCor: correct the effect of guide RNA sequences in clustered regularly interspaced short palindromic repeats/Cas9 screening by machine learning algorithm.

J Genet Genomics. 2020 Nov 20;47(11):672-680. doi: 10.1016/j.jgg.2020.10.007. Epub 2020 Nov 28.

Battling CRISPR-Cas9 off-target genome editing.

Cell Biol Toxicol. 2019 Oct;35(5):403-406. doi: 10.1007/s10565-019-09485-5. Epub 2019 Jul 16.

Minimizing off-target effects in CRISPR-Cas9 genome editing.

Cell Biol Toxicol. 2019 Oct;35(5):399-401. doi: 10.1007/s10565-019-09486-4. Epub 2019 Jul 17.

Engineering guide RNA to reduce the off-target effects of CRISPR.

J Genet Genomics. 2019 Nov 20;46(11):523-529. doi: 10.1016/j.jgg.2019.11.003. Epub 2019 Nov 23.

CRISPR/Cas9 for plant genome editing: accomplishments, problems and prospects.

Plant Cell Rep. 2016 Jul;35(7):1417-27. doi: 10.1007/s00299-016-1985-z. Epub 2016 Apr 25.

PEG-Delivered CRISPR-Cas9 Ribonucleoproteins System for Gene-Editing Screening of Maize Protoplasts.

Genes (Basel). 2020 Sep 2;11(9):1029. doi: 10.3390/genes11091029.

Application of CRISPR-Cas9-Mediated Genome Editing for the Treatment of Myotonic Dystrophy Type 1.

Mol Ther. 2020 Dec 2;28(12):2527-2539. doi: 10.1016/j.ymthe.2020.10.005. Epub 2020 Oct 14.

引用本文的文献

LIPID DROPLET PROTEIN OF SEEDS is involved in the control of lipid droplet size in Arabidopsis seeds and seedlings.

Plant Cell. 2025 May 9;37(5). doi: 10.1093/plcell/koaf121.

Development of an efficient and heritable virus-induced genome editing system in .

Hortic Res. 2024 Dec 28;12(4):uhae364. doi: 10.1093/hr/uhae364. eCollection 2025 Apr.

Critical considerations and computational tools in plant genome editing.

Heliyon. 2024 Dec 14;11(1):e41135. doi: 10.1016/j.heliyon.2024.e41135. eCollection 2025 Jan 15.

Advancements in Plant Gene Editing Technology: From Construct Design to Enhanced Transformation Efficiency.

Biotechnol J. 2024 Dec;19(12):e202400457. doi: 10.1002/biot.202400457.

Engineering cold resilience: implementing gene editing tools for plant cold stress tolerance.

Planta. 2024 Nov 23;261(1):2. doi: 10.1007/s00425-024-04578-w.

Possibility of genome editing for melon breeding.

Breed Sci. 2024 Mar;74(1):47-58. doi: 10.1270/jsbbs.23074. Epub 2024 Apr 9.

Codon usage and expression-based features significantly improve prediction of CRISPR efficiency.

NPJ Syst Biol Appl. 2024 Sep 3;10(1):100. doi: 10.1038/s41540-024-00431-8.

Nuclear pyruvate dehydrogenase complex regulates histone acetylation and transcriptional regulation in the ethylene response.

Sci Adv. 2024 Jul 26;10(30):eado2825. doi: 10.1126/sciadv.ado2825.

Strong association between genomic 3D structure and CRISPR cleavage efficiency.

PLoS Comput Biol. 2024 Jun 7;20(6):e1012214. doi: 10.1371/journal.pcbi.1012214. eCollection 2024 Jun.

Do DEEPER ROOTING 1 Homologs Regulate the Lateral Root Slope Angle in Cucumber ()?

Int J Mol Sci. 2024 Feb 6;25(4):1975. doi: 10.3390/ijms25041975.

本文引用的文献

CRISPR-P 2.0: An Improved CRISPR-Cas9 Tool for Genome Editing in Plants.

Mol Plant. 2017 Mar 6;10(3):530-532. doi: 10.1016/j.molp.2017.01.003. Epub 2017 Jan 13.

CCTop: An Intuitive, Flexible and Reliable CRISPR/Cas9 Target Prediction Tool.

PLoS One. 2015 Apr 24;10(4):e0124633. doi: 10.1371/journal.pone.0124633. eCollection 2015.

GUIDE-seq enables genome-wide profiling of off-target cleavage by CRISPR-Cas nucleases.

Nat Biotechnol. 2015 Feb;33(2):187-197. doi: 10.1038/nbt.3117. Epub 2014 Dec 16.

CHOPCHOP: a CRISPR/Cas9 and TALEN web tool for genome editing.

Nucleic Acids Res. 2014 Jul;42(Web Server issue):W401-7. doi: 10.1093/nar/gku410. Epub 2014 May 26.

CRISPR/Cas9 systems have off-target activity with insertions or deletions between target DNA and guide RNA sequences.

Nucleic Acids Res. 2014 Jun;42(11):7473-85. doi: 10.1093/nar/gku402. Epub 2014 May 16.

Genome-wide prediction of highly specific guide RNA spacers for CRISPR-Cas9-mediated genome editing in model plants and major crops.

Mol Plant. 2014 May;7(5):923-6. doi: 10.1093/mp/ssu009. Epub 2014 Jan 30.

Cas-OFFinder: a fast and versatile algorithm that searches for potential off-target sites of Cas9 RNA-guided endonucleases.

Bioinformatics. 2014 May 15;30(10):1473-5. doi: 10.1093/bioinformatics/btu048. Epub 2014 Jan 24.

CasOT: a genome-wide Cas9/gRNA off-target searching tool.

Bioinformatics. 2014 Apr 15;30(8):1180-1182. doi: 10.1093/bioinformatics/btt764. Epub 2014 Jan 2.

High-frequency off-target mutagenesis induced by CRISPR-Cas nucleases in human cells.

Nat Biotechnol. 2013 Sep;31(9):822-6. doi: 10.1038/nbt.2623. Epub 2013 Jun 23.

Multiplex genome engineering using CRISPR/Cas systems.

Science. 2013 Feb 15;339(6121):819-23. doi: 10.1126/science.1231143. Epub 2013 Jan 3.

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍。

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

文档翻译

学术文献翻译模型，支持多种主流文档格式。

立即体验

CRISPR-PLANT v2: an online resource for highly specific guide RNA spacers based on improved off-target analysis.

作者信息

机构信息

出版信息

相似文献

引用本文的文献

本文引用的文献

文献AI研究员

用中文搜PubMed

文档翻译

Suppr 超能文献

相似文献

引用本文的文献

本文引用的文献