Stambaugh Alexandra, Parks Joshua W, Stott Matthew A, Meena Gopikrishnan G, Hawkins Aaron R, Schmidt Holger
School of Engineering, University of California, Santa Cruz, 1156 High St., Santa Cruz, CA 95064, USA.
ECEn Department, Brigham Young University, 459 Clyde Building, Provo, UT 84602, USA.
Biomed Opt Express. 2018 Jul 16;9(8):3725-3730. doi: 10.1364/BOE.9.003725. eCollection 2018 Aug 1.
The recent massive Zika virus (ZIKV) outbreak illustrates the need for rapid and specific diagnostic techniques. Detecting ZIKV in biological samples poses unique problems: antibody detection of ZIKV is insufficient due to cross-reactivity of Zika antibodies with other flaviviruses, and nucleic acid and protein biomarkers for ZIKV are detectable at different stages of infection. Here, we describe a new optofluidic approach for the parallel detection of different molecular biomarkers using multimode interference (MMI) waveguides. We report differentiated, multiplex detection of both ZIKV biomarker types using multi-spot excitation at two visible wavelengths with over 98% fidelity by combining several analysis techniques.
近期大规模的寨卡病毒（ZIKV）爆发表明需要快速且特异的诊断技术。在生物样本中检测寨卡病毒存在独特的问题：由于寨卡病毒抗体与其他黄病毒的交叉反应性，寨卡病毒的抗体检测并不充分，并且寨卡病毒的核酸和蛋白质生物标志物在感染的不同阶段均可检测到。在此，我们描述了一种使用多模干涉（MMI）波导并行检测不同分子生物标志物的新型光流体方法。我们通过结合多种分析技术，报告了在两个可见波长下利用多点激发对两种寨卡病毒生物标志物类型进行的差异化多重检测，保真度超过98%。
