Yousefshahi Hazhir, Aminsobhani Mohsen, Shokri Mehdi, Shahbazi Razieh
Dental school, Aja University of Medical Sciences, Tehran, Iran.
Department of Dental Biomaterials, Dental School, Shahid Beheshti University of Medical Sciences, Tehran, Iran.
Eur J Transl Myol. 2018 Jul 10;28(3):7545. doi: 10.4081/ejtm.2018.7545.
Prevention of bacterial growth among root canal treatment sessions is a prerequisite for successful root canal treatment. The most common way to achieve this is to use calcium hydroxide in the treatment sessions. Some studies have shown calcium hydroxide inefficiency in this field. The aim of this study was to investigate and compare the effects of silver, copper, zinc oxide and magnesium oxide nanoparticles on the inhibitory effects of calcium hydroxide based on Enterococcus faecalis species. Enterococcus faecalis bacteria having 0.5 McFarland concentration were prepared. Plates containing BHI agar medium were prepared. In each plate, four wells were created and the plate was cultured using a sterile swab. Afterwards, calcium hydroxide composition of 1% and 2% concentration from silver, copper, zinc oxide and magnesium oxide nanoparticles were prepared separately, as well as the combination of calcium hydroxide with 1% silver in combination with 1% of copper, zinc oxide and magnesium oxide nanoparticles, which were then transferred to the wells. After 24 hours of incubation, the inhibition zone diameter was measured. Data were analyzed by Mann-Withney test. At 1% concentration, only the combination of copper nanoparticles with calcium hydroxide could significantly create an inhibition zone larger than calcium hydroxide alone (P value <0.5). At 2% concentration, the combination of copper nanoparticles with calcium hydroxide, and the combination of silver nanoparticles with calcium hydroxide, were significantly higher than calcium hydroxide alone (P value <0.5). The calcium hydroxide composition containing 1% silver nanoparticles in combination with 1% copper, zinc oxide and magnesium oxide nanoparticles significantly increased the growth inhibition zone more than calcium hydroxide alone. (P Value <0.5). Copper nanoparticles showed the best antibacterial properties among silver, copper, magnesium oxide and zinc oxide nanoparticles in combination with calcium hydroxide. Also, the combination of 1% of nanoparticles with each other increases antibacterial properties.
在根管治疗疗程之间预防细菌生长是成功进行根管治疗的前提条件。实现这一点最常用的方法是在治疗疗程中使用氢氧化钙。一些研究表明氢氧化钙在该领域效果不佳。本研究的目的是基于粪肠球菌种属，研究并比较银、铜、氧化锌和氧化镁纳米颗粒对氢氧化钙抑菌作用的影响。制备了浓度为0.5麦氏单位的粪肠球菌。制备了含有脑心浸液琼脂培养基的培养皿。在每个培养皿中创建四个孔，并用无菌拭子对培养皿进行接种培养。之后，分别制备了浓度为1%和2%的银、铜、氧化锌和氧化镁纳米颗粒与氢氧化钙的组合物，以及1%银与1%铜、氧化锌和氧化镁纳米颗粒的氢氧化钙组合物，然后将其转移至孔中。培养24小时后，测量抑菌圈直径。数据采用曼-惠特尼检验进行分析。在1%浓度下，只有铜纳米颗粒与氢氧化钙的组合物能显著产生比单独使用氢氧化钙更大的抑菌圈（P值<0.5）。在2%浓度下，铜纳米颗粒与氢氧化钙的组合物以及银纳米颗粒与氢氧化钙的组合物显著高于单独使用氢氧化钙（P值<0.5）。含有1%银纳米颗粒与1%铜、氧化锌和氧化镁纳米颗粒的氢氧化钙组合物比单独使用氢氧化钙显著增加了生长抑制圈（P值<0.5）。在与氢氧化钙组合的银、铜、氧化镁和氧化锌纳米颗粒中，铜纳米颗粒表现出最佳的抗菌性能。此外，1%的纳米颗粒相互组合可增强抗菌性能。
