Soleimanzadeh Ali, Kian Mehdi, Moradi Sajjad, Malekifard Farin
Department of Theriogenology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Department of Pathobiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran.
Avicenna J Phytomed. 2018 Sep-Oct;8(5):448-456.
Exposure to heavy metals such as lead (Pb) results in oxidative stress induction in the male reproductive system. Herbal medicine can be utilized as antioxidant agents against oxidative stress. (QB) has shown antioxidant activity in previous studies. The aim of the present study was to evaluate effects of QB hydro-alcoholic extract against Pb-induced oxidative stress in the male mice reproductive system.
Forty-two NMRI adult male mice were randomly divided into 7 groups of 6 animals each. Group I was the control group that received no treatment. Group II was the sham group and received 0.2 ml distilled water. Groups III and IV received QB hydro-alcoholic extract 500 and 1000 mg/kg bw, respectively. Group V received Pb 1000 ppm/kg bw. Group VI and VII received Pb 1000 ppm/kg bw and QB extract 500 and 1000 mg/kg bw, respectively. All groups received treatment via oral gavage. After 35 days, sperm parameters (i.e. sperm motility, count and morphology) were evaluated. Levels of sex hormones including LH, FSH, and testosterone, total antioxidant capacity (TAC), superoxide dismutase (SOD) and malondialdehyde (MDA) were measured in animals' serum.
Exposure to Pb negatively affected sperm parameters (i.e. sperm motility, count and morphology), decreased serum concentrations of sex hormones (i.e. LH, FSH, and testosterone), TAC and SOD activity but increased MDA levels. However, co-administration of 500 and 1000 mg/kg bw QB hydro-alcoholic extract and Pb considerably improved sperm parameters (i.e. sperm motility, count and morphology), increased sex hormones (i.e.LH, FSH, and testosterone), TAC, and SOD activity while decreased MDA levels in animals' serum.
Administration of QB extracts (Low dose and high dose) is able to protect the male reproductive system of mice against Pb-induced oxidative stress.
接触铅（Pb）等重金属会导致雄性生殖系统产生氧化应激。草药可作为抗氧化剂抵御氧化应激。（QB）在先前研究中已显示出抗氧化活性。本研究的目的是评估QB水醇提取物对铅诱导的雄性小鼠生殖系统氧化应激的影响。
42只成年NMRI雄性小鼠随机分为7组，每组6只。第一组为对照组，不接受任何处理。第二组为假手术组，接受0.2 ml蒸馏水。第三组和第四组分别接受500和1000 mg/kg体重的QB水醇提取物。第五组接受1000 ppm/kg体重的铅。第六组和第七组分别接受1000 ppm/kg体重的铅和500及1000 mg/kg体重的QB提取物。所有组均通过口服灌胃给药。35天后，评估精子参数（即精子活力、数量和形态）。检测动物血清中性激素（包括促黄体生成素、促卵泡生成素和睾酮）水平、总抗氧化能力（TAC）、超氧化物歧化酶（SOD）和丙二醛（MDA）水平。
接触铅对精子参数（即精子活力、数量和形态）产生负面影响，降低了血清中性激素（即促黄体生成素、促卵泡生成素和睾酮）浓度、TAC和SOD活性，但增加了MDA水平。然而，同时给予500和1000 mg/kg体重的QB水醇提取物和铅可显著改善精子参数（即精子活力、数量和形态），增加性激素（即促黄体生成素、促卵泡生成素和睾酮）、TAC和SOD活性，同时降低动物血清中的MDA水平。
给予QB提取物（低剂量和高剂量）能够保护小鼠雄性生殖系统免受铅诱导的氧化应激。
